Evaluation of mineral content loss in artificial caries lesions by polarized light microscopy analysis in relation to microhardness

• Grant number: 06/05277-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2007
• End date: January 31, 2009
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Mônica Campos Serra
• Grantee: Francisco Carlos Rehder Neto
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Dental caries involves a diffusion process of organic acids into the mineralized tissues. These acids may be neutralized, resulting in pH alterations, which can lead to caries lesions development, modifying the mineral content of these tissues. The aim of this study will be to evaluate in vitro caries-like lesions formation in human and bovine dental substrates through polarized light microscopy and microhardness, whose relationship will be also tested. Dentine and enamel slabs will be obtained from human and bovine teeth. An induction model of artificial caries will be applied. The number of pH cycles, time of demineralization and remineralization, and volume of solution will be defined in prelimary tests. At the end of the stage of caries-like lesion induction, specimens will be sectioned in two parts. One section will be assessed for Knoop microhardness and the other one will be analyzed by polarized light microscopy. After veryfing homoscedasticity and normality, Analysis of Variance will be performed. Otherwise, non-parametric methods will be used. Analysis will be accomplished through a statistical package. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REHDER NETO, F. C.; MAEDA, F. A.; TURSSI, C. P.; SERRA, M. C.. Potential agents to control enamel caries-like lesions. Journal of Dentistry, v. 37, n. 10, p. 786-790, OCT 2009. (06/05277-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

REHDER NETO, Francisco Carlos. Progression and inhibition of artifical caries lesions in human and bovine substrate evaluated by surface microhardness. 2009. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.