Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis

• Grant number: 07/08210-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2008
• End date: December 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Daniel Araki Ribeiro
• Grantee: Ana Carolina Cuzzuol Fracalossi
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis, AP.JP

Abstract

While it has been clearly demonstrated the role of functional genomics on tumorigenesis, the biological behavior of some oncogenes, tumoral invasiveness, DNA repair kinetics and content along time course oral cancer progression are not fully established. Thus far, the goal of this project will be to investigate the role of some oncogenes, cellular proliferation control, DNA repair kinetics and content during experimental rat oral carcinogenesis. In this regard, a total of 30 male Wistar rats will be exposed to carcinogen 4-nitroquinoline 1-oxide by drinking water at 50 ppm dose and then, they will be sacrificed at four, 12 and 20 weeks post-treatment. A total of 30 rats will be used as negative control. Herein, it will be evaluated mutations in H-ras and K-ras oncogenes, expression of p21RAS, ki-67 e alpha-SMA, through immunohistochemistry and global genome content and DNA repair system performance by single cell gel (comet) assay during rat tongue carcinogenesis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CUZZUOL FRACALOSSI, ANA CAROLINA; SILVA, MARCELO DE SOUZA; FUJIYAMA OSHIMA, CELINA TIJUKO; RIBEIRO, DANIEL ARAKI. Wnt/beta-catenin signalling pathway following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. Experimental and Molecular Pathology, v. 88, n. 1, p. 176-183, FEB 2010. (07/01228-4, 07/08210-3)

CUZZUOL FRACALOSSI, ANA CAROLINA; MIRANDA, SANDRA REGINA; FUJIYAMA OSHIMA, CELINA TIJUKO; FRANCO, MARCELLO; RIBEIRO, DANIEL ARAKI. The role of matrix metalloproteinases 2 and 9 during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. Journal of Molecular Histology, v. 41, n. 1, p. 19-25, FEB 2010. (07/01228-4, 07/08210-3)

RIBEIRO, DANIEL ARAKI; CUZZUOL FRACALOSSI, ANA CAROLINA; UATARI, SILVIA AKEMI; FUJIYAMA OSHIMA, CELINA TIZUKO; FAVERO SALVADORI, DAISY MARIA. Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide. IN VIVO, v. 23, n. 6, p. 937-942, NOV-DEC 2009. (07/01228-4, 07/08210-3)