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Effects of intrapulpal pressure on the long-term bond strength and nanoleakage of resin-dentin interfaces produced by self-adhesive cements: an in vitro study

Grant number: 08/05179-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2009
Effective date (End): January 31, 2010
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:André Figueiredo Reis
Grantee:Rodrigo Sversut de Alexandre
Host Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil


The aim of this in vitro study is to evaluate the effects of intrapulpal pressure and storage time on the bond strength and nanoleakage patterns of resin-dentin interfaces produced by different self-adhesive cements. Three self-adhesive cements will be used: RelyX Unicem (3M Espe), BisCem (Bisco), Maxcem (Kerr); and two conventional resin cements: Rely X ARC (3M Espe), Panavia F (Kuraray). One hundred human third molars will be used. Teeth will be randomly distributed in 5 groups according to the luting materials and then will be assigned to 4 subgroups, according to the presence or not of positive pulpal pressure and water storage time: 24 hours and 12 months. Discs of pre-polymerized composite resin will be cemented according to manufacturers' instructions. After the storage periods, the restored teeth (n=4) will be serially sectioned, producing beams with a cross-sectional area of 0.8 mm2 at the resin-dentin interface, which will be tested in tension in a universal testing machine. Teeth luted with each material will be submitted to a nanoleakage protocol with AgNO3 after each storage period (n=5) and will be prepared for observation in a Scanning and Transmission Electron Microscope. The bond strength and nanoleakage data will be statistically analyzed by two-way ANOVA and Tukey test.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA-FILHO, R. C.; ELY, C.; AMARAL, R. C.; RODRIGUES, J. A.; ROULET, J-F; CASSONI, A.; REIS, A. F.. Effect of Different Adhesive Systems Used for Immediate Dentin Sealing on Bond Strength of a Self-Adhesive Resin Cement to Dentin. Operative Dentistry, v. 43, n. 4, p. 391-397, . (08/07628-7, 09/16261-2, 14/23401-3, 08/05179-0, 07/06083-4)
SANTANA, V. B.; DE ALEXANDRE, R. S.; RODRIGUES, J. A.; ELY, C.; REIS, A. F.. Effects of Immediate Dentin Sealing and Pulpal Pressure on Resin Cement Bond Strength and Nanoleakage. Operative Dentistry, v. 41, n. 2, p. 189-199, . (08/07628-7, 09/16261-2, 14/23401-3, 08/05179-0, 07/06083-4)
DE ALEXANDRE, R. S.; SANTANA, V. B.; KASAZ, A. C.; ARRAIS, C. A. G.; RODRIGUES, J. A.; REIS, A. F.. Effect of Long-term Simulated Pulpal Pressure on the Bond Strength and Nano leakage of Resin-luting Agents With Different Bonding Strategies. Operative Dentistry, v. 39, n. 5, p. 508-520, . (08/05179-0, 09/16261-2)

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