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Impact of remineralizing agents on the resin/caries-affected dentin bond longevity: bond strength and nanoleakage

Grant number: 15/24927-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2016
Effective date (End): August 31, 2020
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Regina Maria Puppin Rontani
Grantee:Kelly Maria Silva Moreira
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


This study will determine the longevity of the bond Resin/Dentin Affected by Caries (DAC) after remineralizing treatment of NaF 0.2%, CPP-ACP or CurodontTM Repair and AdperTM Single Bond 2 adhesive system, through the bond strength for microtensile and nanoleakage, after 24h, 6 and 18 months of storage in Simulated Body Fluid (SBF), with pulp pressure simulation. The sample will consist of 165 non-carious third molars and the DAC will be produced by biological method. The teeth will be randomly divided into 5 groups (n=8) according to the treatment of dentin surface: G1: Sound dentin; G2: DAC; G3: DAC + NaF 0.2%; G4: DAC + CPP-ACP; G5: DAC + CurodontTM Repair. In sequence, the dentin will be submitted to union with AdperTM Single Bond 2 and FiltekTM Z350 resin block (H4mm/height) will be performed on it. The tooth/restoration sets are randomly distributed depending on the storage time in SBF at 37°C, with the pulp pressure simulation. After 24h, 6 and 18 months, these sets will be sectioned in the form of stick (average areaH1,0 mm2) to microtensile and in the longitudinal direction (2 central slices/tooth) to nanoinfiltration. The specimens will be submitted to microtensile and nanoleakage. The fracture patterns and the union interface will be analyzed in scanning electron microscopy. The data of microtensile strength will be submitted to factorial ANOVA and Tukey and the data obtained in the assessment of fracture pattern to the Kruskal-Wallis test (±=5%). Descriptive analysis will be performed to nanoinfiltação and formation/degradation of the hybrid layer.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOREIRA, KELLY MARIA; BERTASSONI, LUIZ EDUARDO; DAVIES, ROBERT PHILL; JOIA, FELIPE; HOFLING, JOSE FRANCISCO; NASCIMENTO, FABIO DUPRAT; PUPPIN-RONTANI, REGINA MARIA. Impact of biomineralization on resin/biomineralized dentin bond longevity in a minimally invasive approach: An "in vitro" 18-month follow-up. Dental Materials, v. 37, n. 5, p. 14-pg., . (15/24927-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MOREIRA, Kelly Maria Silva. A long-term ''in vitro study on resin/biomineralized dentin bonding. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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