Expression, pufification and structural characterization of proteins coded by two plant viruses.

• Grant number: 10/00211-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2010
• End date: February 29, 2012
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Raghuvir Krishnaswamy Arni
• Grantee: Livia José Regatieri
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Viral replication involves virus entry in plant cell and hijacking the host machinery for necessary protein production to give rise new viral particles. Molecular studies of plant viruses have been made, for example, RNAs sequencing, subgenomic RNA detection and determination of strategy for expression of the virus genome. However, several aspects related to the function and structure of some proteins, as well as its relation to the viral replication cycle, are still unknown. Thus, the aim of this study is a high-scale production and a high-purity purification of viral proteins in its native state. The proteins will be expressed in Escherichia coli, aiming the high-resolution determination of its three-dimensional structures, providing relevant informations about their roles and functions. The following proteins will be studied: Cole latent virus capsidial protein (CP), Pepper ringspot virus RNA-dependent RNA polymerase (RdRP) and Pepper ringspot movement protein (MP).

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

REGATIERI, Livia José. Expressão, purificação e caracterização estrutural de proteínas codificadas por dois fitovírus. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.