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Assessment of cellular function of leukocytes by flow cytometry and the influence of polychlorinated biphenyls in the development of fibropapillomatosis in Chelonia mydas (Linnaeus, 1758) (Testudines, Cheloniidae)

Grant number: 10/01781-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2010
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Ecology
Principal Investigator:Eliana Reiko Matushima
Grantee:Silmara Rossi
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Chelonia mydas, called green sea turtle, feeds and nests in Brazilian coast. The fibropapillomatosis, disease characterized by benign skin tumors (fibropapillomas), is one of the most threats against this species. Studies suggest relations between a specific virus and environmental and genetic factors. Will be studied green sea turtles with and without fibropapillomas from Brazilian areas. Biometric data, numbers and distributions of tumors by anatomic regions will be noted and will be take blood sample. The aims are (1) to asses leukocyte activity by flow cytometry: Phagocytosis stimulated by Zymosan A (Saccharomyces cerevisiae) Bio Particles®, Alexa Fluor® 594 conjugate and oxidative burst - basal and stimulated by PMA (phorbol miristate-acetate);(2) to investigate polychorinated biphenyls (PCBs) in blood samples; (3) to establish correlations between PCBs and cell activity of non-affected and affected turtles from study areas. The fibropapillomatosis is still unclear and affect many juvenils green sea turtles. Diseases related with pollution are a relevant factor for the ecosystem imbalance.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROSSI, SILMARA; SANCHEZ-SARMIENTO, ANGELICA MARIA; DOS SANTOS, ROBSON GUIMARAES; ZAMANA, ROBERTA RAMBLAS; SETIM PRIOSTE, FABIOLA ELOISA; GATTAMORTA, MARCO AURELIO; CRUZ OCHOA, PABLO FELIPE; HILDEBRAND GRISI-FILHO, JOSE HENRIQUE; MATUSHIMA, ELIANA REIKO. Monitoring green sea turtles in Brazilian feeding areas: relating body condition index to fibropapillomatosis prevalence. Journal of the Marine Biological Association of the United Kingdom, v. 99, n. 8, p. 1879-1887, DEC 2019. Web of Science Citations: 0.
SANCHEZ-SARMIENTO, ANGELICA MARIA; ROSSI, SILMARA; VILCA, FRANZ ZIRENA; THIJL VANSTREELS, RALPH ERIC; MONTEIRO, SERGIO HENRIQUE; VALE, LUIZ AMERICO S.; DOS SANTOS, ROBSON GUIMARAES; MARIGO, JULIANA; BERTOZZI, CAROLINA PACHECO; HILDEBRAND GRISI FILHO, JOSE HENRIQUE; TORNISIELO, VALDEMAR LUIZ; MATUSHIMA, ELIANA REIKO. Organochlorine pesticides in green sea turtles (Chelonia mydas) with and without fibropapillomatosis caught at three feeding areas off Brazil. Journal of the Marine Biological Association of the United Kingdom, v. 97, n. 1, p. 215-223, FEB 2017. Web of Science Citations: 3.
ROSSI, SILMARA; SANCHEZ-SARMIENTO, ANGELICA MARIA; THIJL VANSTREELS, RALPH ERIC; DOS SANTOS, ROBSON GUIMARAES; SETIM PRIOSTE, FABIOLA ELOISA; GATTAMORTA, MARCO AURELIO; HILDEBRAND GRISI-FILHO, JOSE HENRIQUE; MATUSHIMA, ELIANA REIKO. Challenges in Evaluating the Severity of Fibropapillomatosis: A Proposal for Objective Index and Score System for Green Sea Turtles (Chelonia mydas) in Brazil. PLoS One, v. 11, n. 12 DEC 9 2016. Web of Science Citations: 4.
ROSSI, SILMARA; DE QUEIROZ HAZARBASSANOV, NICOLLE G. T.; SANCHEZ-SARMIENTO, ANGELICA MARIA; SETIM PRIOSTE, FABIOLA ELOISA; MATUSHIMA, ELIANA REIKO. Immune Response of Green Sea Turtles with and without Fibropapillomatosis: Evaluating Oxidative Burst and Phagocytosis via Flow Cytometry. CHELONIAN CONSERVATION AND BIOLOGY, v. 15, n. 2, p. 273-278, DEC 2016. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROSSI, Silmara. Analysis of leukocyte activity and polychlorinated biphenyls applied to the study of fibropapillomatosis in Chelonia mydas (Testudines, Cheloniidae) (Linnaeus 1758). 2014. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz Piracicaba.

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