Allelic variation in sugarcane: quantification of sequence polymorphism

• Grant number: 10/02610-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2010
• End date: August 31, 2012
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Michel Georges Albert Vincentz
• Grantee: Mariane de Mendonça Vilela
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Sugarcane belongs to the genus Saccharum L., and had been originated in Southeast Asia, where since ancient times has been used for the production of sugar. Bioethanol, a byproduct of sugar cane, is an important source of renewable and is less polluting energy than petroleum derivatives. So far, the genetic improvement of sugarcane have been based essencially on breeding and selection of new varieties originated from crosses between hybrids of S. officinarum and S. spontaneum. New genomic resources have been developed to help genetic research in sugarcane: genetic maps, collections of ESTs and BACs are already available. However the high polyploidy, the heterozygosity and the irregular meiotic behavior has become a challenge to the application of molecular markers and genetic studies in general. As one of the most complex genomes that is known, the synteny observed with related genera has become another essential tool for understanding the genome of sugarcane. Given the growing economic importance and the intriguing complexity of the genome of sugarcane, we propose to sequence and annotate homo/homeólogas regions of tree single copy genes Leafy, TOR kinase and Phytochrome C in order to assess the allelic variation and the expression homeostasis of these genes in segregating populations. The knowledge gained should help reveal new aspects of the evolution of the genome of sugarcane as well as upgrade more accurately the improvement process of this plant. The project also fits within the context of an international effort to sequence the genome of sugarcane. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILELA, MARIANE DE MENDONCA; DEL BEM, LUIZ EDUARDO; VAN SLUYS, MARIE-ANNE; DE SETTA, NATHALIA; KITAJIMA, JOAO PAULO; QUEIROGA CRUZ, GUILHERME MARCELO; SFORCA, DANILO AUGUSTO; DE SOUZA, ANETE PEREIRA; GOMES FERREIRA, PAULO CAVALCANTI; GRATIVOL, CLICIA; et al. Analysis of Three Sugarcane Homo/Homeologous Regions Suggests Independent Polyploidization Events of Saccharum officinarum and Saccharum spontaneum. GENOME BIOLOGY AND EVOLUTION, v. 9, n. 2, p. 266-278, FEB 2017. (10/02610-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VILELA, Mariane de Mendonça. Compreendendo os genomas poliploides: 'insights' sobre a organização e evolução do genoma da cana-de-açúcar. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.