| Grant number: | 19/21682-9 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Effective date (Start): | September 01, 2020 |
| Effective date (End): | August 31, 2024 |
| Field of knowledge: | Biological Sciences - Genetics - Plant Genetics |
| Principal Investigator: | Anete Pereira de Souza |
| Grantee: | Ricardo José Gonzaga Pimenta |
| Host Institution: | Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated scholarship(s): | 21/11449-5 - Developing broad-spectrum resistance to turnip mosaic virus in rapeseed, BE.EP.DD |
Abstract Sugarcane (Saccharum spp.) Cultivation is of unquestionable importance in the Brazilian economy, totalling more than $ 11 billion in exports in 2016. The improvement of this crop is marked by its genetic complexity - in addition to its highly polyploid genome. Often has aneuploidies, which makes research difficult in the area and contributes to the development of new cultivars is a long and challenging process. One of the main viruses affecting this crop is the sugarcane leaf yellowing caused by the homonymous virus (SCYLV). The most common symptoms resulting from infection are yellowing of the leaf veins and leaf chlorosis, but asymptomatic cases are frequent. In the 1990s, leaf yellowing caused losses of 50% of Brazilian sugarcane production, and currently, its causal agent is endemic in the primary sugar and alcohol regions of Brazil and the world. As a result, resistance to SCYLV is of high relevance in sugarcane breeding, but few genetic studies have investigated this subject in-depth, especially in Brazil. The present project proposes, therefore, the application of different approaches based on new generation sequencing seeking to elucidate the genetic basis of resistance to this virus. First, a wide genomic association study will be conducted in a sugarcane panel of the Campinas Agronomic Institute. Plant resistance of this panel to SCYLV will be determined according to the manifestation of disease symptoms and viral quantification in the plants. The accessions will be genotyped using sequencing genotyping technology, which will allow the discovery of a large number of markers with allelic dosage information. Second, an experiment will be conducted to analyse the transcriptional response of two sugarcane cultivars - one susceptible and one resistant to the virus - in response to SCYLV infection. To this end, pathogen-free plants will be infected with the virus and have their transcriptome sequenced by RNA-Seq. Using these two methodologies, we intend to identify molecular markers applicable to sugarcane breeding and also genes whose expression is associated with the response to SCYLV, contributing to the understanding of the processes involved in virus resistance. (AU) | |
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