Development of an enzymatic cocktail for application in biorefinery

• Grant number: 10/10184-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2010
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Liliane Fraga Costa Ribeiro
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 08/57908-6 - National Institute of Science and Technology of Bioethanol, AP.BIOEN.TEM

Abstract

The use of ethanol as fuel has advantages as it is an inexhaustible energy source and because ethanol combustion lead to reduced CO2 emissions compared to fossil fuels. The production of bioethanol mainly uses molasses from sugar cane, discarding the pulp. The pulp, or bagasse, is a residue rich in cellulose and hemicellulose, that is why it can also be used for ethanol production, the so-called second generation ethanol. Thus, this work aims to develop an enzymatic cocktail containing cellulolytic and xylanolytic enzymes that can be used on bagasse pre-treated, releasing sugars that can be used by yeasts, such as Saccharomyces, for the production of bioethanol. The use of an enzymatic mix, using enzymes that will be cloned and expressed in filamentous fungi, and the fact they can be reused, because it will be immobilized in chemical supports, is an innovation in the research of our group and therefore has much to contribute to scientific growth in our laboratory. This project is inserted into the theme promoted by FAPESP, National Institute of Biotechnology for Ethanol, under the coordination of Professor Dr. Marcos Silveira Buckeridge, in partnership with CNPq - Announcement of the National Science, Technology and Innovation.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, LUCAS FERREIRA; TULLMAN, JENNIFER; NICHOLES, NATHAN; BERGAMACHI SILVA, SERGIO RUSCHI; VIEIRA, DAVI SERRADELLA; OSTERMEIER, MARC; WARD, RICHARD JOHN. A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination. BIOTECHNOLOGY FOR BIOFUELS, v. 9, JUN 6 2016. (10/18850-2, 10/10184-3, 10/07133-8)

RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, JUL 29 2014. (10/18850-2, 12/12859-3, 13/18910-3, 10/07133-8, 10/10184-3, 12/02017-5, 08/57908-6)

VITCOSQUE, GABRIELA LEAL; RIBEIRO, LILIANE FRAGA COSTA; DE LUCAS, ROSYMAR COUTINHO; DA SILVA, TONY MARCIO; RIBEIRO, LUCAS FERREIRA; DE LIMA DAMASIO, ANDRE RICARDO; FARINAS, CRISTIANE SANCHEZ; LOPES GONCALVES, ALINE ZORZETTO; SEGATO, FERNANDO; BUCKERIDGE, MARCOS SILVEIRA; et al. The functional properties of a xyloglucanase (GH12) of Aspergillus terreus expressed in Aspergillus nidulans may increase performance of biomass degradation. Applied Microbiology and Biotechnology, v. 100, n. 21, p. 9133-9144, NOV 2016. (10/10184-3, 14/18714-2, 12/20549-4)