STUDY OF EFFECT OF THE INTESTINAL ISCHEMIA AND REPERFUSION ON PURINE P2X7 RECEPTOR AND ENTERIC NERVOUS SYSTEM OF THE RATS ILEUM

• Grant number: 10/10510-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2010
• End date: July 31, 2014
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Patricia Castelucci
• Grantee: Kelly Palombit
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Abdominal pain may be consequent to several causes, among the various possibilities need to be attentive to the intestinal ischemia. In the digestive tract ischemia / reperfusion (I/R-i) entails morphological changes in enteric neurons. This work aims to analyze the different classes of neuronal and P2X7 receptor in the enteric nervous system of the rats ileum subjected to I/R-i. Intestinal ischemia is obtained by the obstruction of blood flow ileal arteries within 60 minutes followed by reperfusion periods of 4, 24 hours, 7, 14 and 28 days. In the sham group there is no occlusion of the ileal arteries. The tissues will be prepared by immunohistochemical methods for double and triple marks of P2X7 receptor with Nitric Oxide Synthase (NOS), calbindin (Calb), acetylcholine transferase (ChAT), calretinin (Calr), Neuropeptide Y (NPY) and Vasoactive Intestinal Peptide (VIP) and P2X7 receptor, NOS, of ChAT, the Calr, NPY and VIP with anti-pan-neuronal anti-HuC/D. The qualitative and quantitative analysis from of double or triple labelling, the neuronal density and area of neuronal profiles will be obtained from the fluorescence microscopes and/or confocal scanning laser. Also, be made the analysis of protein expression of the P2X7 receptor in different periods of reperfusion, analysis of apoptosis/necrosis analysis of myeloperoxidase, ultrastructural analysis of organelles and analysis of enteric plasticity.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALOMBIT, KELLY; MENDES, CRISTINA EUSEBIO; TAVARES-DE-LIMA, WOTHAN; BARRETO-CHAVES, MARIA LUIZA; CASTELUCCI, PATRICIA. Blockage of the P2X7 Receptor Attenuates Harmful Changes Produced by Ischemia and Reperfusion in the Myenteric Plexus. Digestive Diseases and Sciences, v. 64, n. 7, p. 1815-1829, JUL 2019. (10/10510-8, 12/00259-1, 14/25927-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PALOMBIT, Kelly. Role of the Brilliant Blue G antagonist on the myenteric neurons immunoreactive for the P2X7 receptor of the rats ileum following the intestinal ischemia/reperfusion.. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.