Role of the Brilliant Blue G antagonist on the myenteric neurons immunoreactive for the P2X7 receptor of the rats ileum following the intestinal ischemia/reperfusion.

Author(s)

Kelly Palombit

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-08-22

Examining board members

Patricia Castelucci; Patricia Cristina Baleeiro Beltrao Braga; Silvia Lacchini; Maria Angélica Miglino; Ii Sei Watanabe

• Advisor: Patricia Castelucci

Abstract

We analyzed the effects of BBG antagonist on the P2X7 receptor and rats ileum enteric neurons subjected to I/R. Intestinal ischemia was obtained by ileal vessels obstruction for 45 minutes, followed by reperfusion of 0 h, 24 h and 14 days. The BBG was applied in I/R 24 h and 14 days groups in dosages of 50 and 100 mg/kg. The I/R 0 h is the group without reperfusion. Tissues were prepared by double labeling, western blotting, myeloperoxidase reaction, histology and intestinal motility analyzes. Our results demonstrated the presence of the P2X7 receptor in myenteric neurons. There was a decrease in density and in the area of the cell body profile of the myenteric neurons in the I/R groups and recovery in the BBG groups. In I/R groups there was an increase in the expression of P2X7 receptor and in the number of neutrophils and there was a decrease in intestinal motility and recover in the BBG groups. The results suggest that I/R affect the myenteric neurons and that the BBG may have attenuated the effects of ischemia, thus demonstrating a possible neuroprotection. (AU)

• FAPESP's process: 10/10510-8 - STUDY OF EFFECT OF THE INTESTINAL ISCHEMIA AND REPERFUSION ON PURINE P2X7 RECEPTOR AND ENTERIC NERVOUS SYSTEM OF THE RATS ILEUM
• Grantee: Kelly Palombit
• Support Opportunities: Scholarships in Brazil - Doctorate