Bacterial disease in catfish: isolation, characterization and pathogenesis

• Grant number: 10/14490-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2011
• End date: February 28, 2013
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Fabiana Pilarski
• Grantee: Santiago Benites de Padua
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The first occurrence of infection by Citrobacter freundii, Lactococcus lactis subsp. lactis and Enterococcus faecalis associated with outbreaks of diseases in South American catfish was recorded in this study. The isolates were biochemically characterized and the profile antimicrobial susceptibility performed. For isolation, fingerlings of catfish from sequential outbreaks at different farms nursery in the state of Mato Grosso do Sul and also creations in the laboratory were used. Fingerlings with clinical signs of bacterial infection were necropsied and biopsy of the kidney and brain obtained for bacterial isolation in appropriate culture media. The isolates were subjected to Gram staining, oxidase and catalase test, hemolysis production and presumptive identification using commercial kits API 20E and API 20 Strep. The analysis of susceptibility to 15 antimicrobials was performed by the disc diffusion method with the Muller Hinton Agar. Were obtained two isolates of C. freundii from cachara, which showed multiresistance to 66.7% of the antimicrobials tested. In experimental challenge, the disease was successfully reproduced, characterized as hemorrhagic septicemia, with severe enteritis, and reisolation of C. freundii from infected tissue was obtained. Another 57 isolates obtained from kidney and brain of catfish were characterized as gram-positive cocci associated with meningoencephalitis, identified as Lactococcus lactis subsp. lactis and Enterococcus faecalis. All isolates showed multidrug resistance to antimicrobials. The bacteria L. lactis subsp. lactis was only sensitive to ampicillin and penicillin. All our isolates of E. faecalis were sensitive to sulfazotrim and amoxilin and to clavulanic acid.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALLADAO, GUSTAVO MORAES RAMOS; GALLANI, SILVIA UMEDA; DE PADUA, SANTIAGO BENITES; MARTINS, MAURICIO LATERCA; PILARSKI, FABIANA. Trichodina heterodentata (Ciliophora) infestation on Prochilodus lineatus larvae: a host-parasite relationship study. Parasitology, v. 141, n. 5, p. 662-669, APR 2014. (10/14490-1, 12/19414-7)

RAMOS VALLADAO, GUSTAVO MORAES; GIANNECCHINI, LUIZ GUSTAVO; MARTINS, MAURICIO LATERCA; DE PADUA, SANTIAGO BENITES. Trichodina modesta: exótico ciliado na região Neotropical parasitando hospedeiro não usual. Revista Brasileira de Parasitologia Veterinária, v. 24, n. 2, p. 162-167, APR-JUN 2015. (10/14490-1, 12/19414-7)

RAMOS VALLADAO, GUSTAVO MORAES; GIANNECCHINI, LUIZ GUSTAVO; MARTINS, MAURICIO LATERCA; DE PADUA, SANTIAGO BENITES. Trichodina modesta: an exotic ciliate in the Neotropical region parasitizing an unusual host. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 24, n. 2, p. 6-pg., 2015-04-01. (12/19414-7, 10/14490-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PADUA, Santiago Benites de. Doenças bacterianas em bagres sul americanos: isolamento, caracterização e patogenia. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.