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Evaluation of polymorphisms of genes DGAT1 and TCAP as markers for carcass and meat characteristics in beef cattle Nellore (Bos indicus)

Grant number: 10/17237-5
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2011
Effective date (End): February 28, 2013
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Luis Artur Loyola Chardulo
Grantee:Bárbara Oliveira Borges
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


This project aims to appraise the allele and genotype frequencies of DGAT1-VNTR polymorphisms of the gene DGAT1 and G346A (GenBank: AY428575) of gene TCAP in Nelore beef cattle (Bos indicus), as well as check the occurrence of associations between these polymorphisms and traits related to carcass composition and meat quality in the breed. It will be used about 600 Nelore cattle, feedlot under the age of two years, all with performance and pedigree information available. The selected animals will be slaughtered in a slaughterhouse. The Longissimus dorsi muscle samples will be collected from each animal for determining the quantity and quality of meat produced. For this we will measure the rib eye area, fat thickness, index of marbling, shear force, instrumental color, and chemical analysis of meat as lipid and myofibrillar fragmentation index (MFI). Genotyping of polymorphisms will be performed by PCR in the DGAT1 gene and PCR-RFLP in the gene TCAP. The documentation of the gels for subsequent data analysis will be performed using digital photo documentation system. The genotypes of individuals are determined for each polymorphism by analysis of fragment size in base pairs (bp). From the genotypes identified in the gels will be calculated the allele and genotype frequencies for each of the polymorphisms.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORGES, BARBARA OLIVEIRA; CURI, ROGERIO ABDALLAH; BALDI, FERNANDO; BRAGA FEITOSA, FABIELI LOISE; FERNANDES DE ANDRADE, WILLIAN BRUNO; DE ALBUQUERQUE, LUCIA GALVAO; DE OLIVEIRA, HENRIQUE NUNES; LOYOLA CHARDULO, LUIS ARTUR. Polymorphisms in candidate genes and their association with carcass traits and meat quality in Nellore cattle. Pesquisa Agropecuária Brasileira, v. 49, n. 5, p. 364-371, MAY 2014. Web of Science Citations: 6.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BORGES, Bárbara Oliveira. Marcadores moleculares associados à qualidade de carne em bovinos Nelore. 2013. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias.

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