CAD gene family characterization involved in wood formation tissues in Tectona grandis

• Grant number: 10/19294-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2011
• End date: July 31, 2012
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Helaine Carrer
• Grantee: Tarcísio Sales Vasconcelos
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Teak (Tectona grandis L. f.) has excellent physical and chemical characteristics, which ensure its high quality and durability, making it a target species of great commercial interest. However, two major concerns direct teak market: low growth rates and difficulty to activate the secondary cell formation in the stem. These questions are related to metabolic processes of wood production, involving vascular cambium cell division, cell expansion, secondary cell wall deposition, programmed cell death and compact Wood formation. Several genes are involved in these five steps, but the gene CAD (Cinnamyl alcohol dehydrogenase), (which encodes a protein that catalyzes the production of monolignols, precursors of the final lignin) has already been described in Populus trichocarpa, and shows alignments with appropriate proportions of identity (over 70%) with genes of several forest species. Indeed, the objective of this work is to characterize the CAD gene in formation and secondary growth wood tissues in teak. For the study of this gene, it will be done RNA extraction of plant material from apical buds, secondary xylem and phloem differentiation in teak individuals of different ages (5 months, one year, five years and 10 years), with the aim of isolating the gene CAD and apply molecular biology techniques and bioinformatics tools for the characterization and expression analysis in different stages of plant growth and subsequently diagnose the teak wood characteristics.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GALEANO, ESTEBAN; VASCONCELOS, TARCISIO SALES; DE OLIVEIRA, PERLA NOVAIS; CARRER, HELAINE. Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.). PLoS One, v. 14, n. 9, SEP 9 2019. (13/06299-8, 10/19294-6)

GALEANO, E.; BARROSO, A. A. M.; VASCONCELOS, T. S.; LOPEZ-RUBIO, A.; ALBRECHT, A. J. P.; VICTORIA FILHO, R.; CARRER, H.. EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis. Genetics and Molecular Research, v. 15, n. 3, 2016. (12/10371-3, 13/06299-8, 12/10372-0, 10/19294-6)

GALEANO, ESTEBAN; VASCONCELOS, TARCISIO SALES; CARRER, HELAINE. Characterization of Cinnamyl Alcohol Dehydrogenase gene family in lignifying tissues of Tectona grandis L.f.. SILVAE GENETICA, v. 67, n. 1, p. 1-11, FEB 2018. (13/06299-8, 10/19294-6)