Evaluation of the protease activated receptor 2(PAR-2) expression in chronic periodontitis patients, before and after non-surgical periodontal treatment

• Grant number: 10/20477-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2011
• End date: June 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Marinella Holzhausen Caldeira
• Grantee: Vanessa Tubero Euzebio Alves
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 12/22439-1 - Role of Protease-Activated Receptor 2 (PAR2) during Porphyromonas gingivalis infection on the regulation of p38-MAPK signaling in periodontal cells, BE.EP.DR

Abstract

The association of protease activated receptor 2 (PAR-2) with periodontitis, an oral chronic inflammation that leads to bone loss and consequently to tooth loss, was first suggested by in vitro studies which have demonstrated that osteoblasts, epithelial cells and human gingival fibroblasts express PAR-2. In addition, it was observed that local administration of a PAR-2 selective agonist (SLIGRL) causes gingival granulocyte infiltration and periodontitis in rats. Furthermore, PAR-2 knockout mice (PAR-2-/-) showed less inflammation due to Porphyromonas gingivalis (Pg) infection. In a recent study by our group, we have shown that in chronic periodontitis patients, an increased genetic expression of PAR-2 is associated to the presence of increased levels of pro-inflammatory mediators, total proteolytic activity, proteinase-3, and increased Pg prevalence, from which gingipain is derived. However, these results did not show whether this genetic expression is found increased due to the presence of periodontal disease or due to a constitutive feature that favors the commencement of periodontal disease. Therefore, in this present study, crevicular cells will be evaluated regarding the correlation between genetic and protein expression of PAR-2 (Real Time-PCR, Western blot) and the periodontal clinical parameters in human chronic periodontitis, before and after conventional periodontal treatment.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

ABREU, I. S.; EUZEBIO ALVES, V. T.; BENEDETE, A. P. S.; BUENO DA SILVA, H. A.; FRANCA, B. N.; SARAIVA, L.; LIMA, L. A.; CARVALHO, M. H.; HOLZHAUSEN, M.. Gingival crevicular fluid levels of protease-activated receptors type 1 and type 2 in diabetic patients with periodontitis. JOURNAL OF PERIODONTAL RESEARCH, v. 51, n. 5, p. 577-585, OCT 2016. (09/16373-5, 10/20477-8, 14/06387-7, 10/16605-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Vanessa Tubero Euzebio. Evaluation of the protease activated receptor 2 (PAR2) expression in chronic periodontitis patients, before and after non-surgical periodontal treatment. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.