Evaluation of Microbiological and Chemical Models for the Study of Bio(transformations) of Antibiotic Monensin A

• Grant number: 11/05800-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2011
• Effective date (End): April 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Marilda das Dores Assis
• Grantee: Bruno Alves Rocha
• Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 09/51812-0 - Development of a platform for the study of in vitro and in vivo metabolism of natural products, a need for pre-clinical testing system, AP.BTA.TEM

Abstract

This study aims to use alternative means to study the metabolism of monensin A, an important antibiotic ionophore used in veterinary applications in Brazil and the world against coccidiosis, by applying techniques employing biomimetic metabolism (metalloporphyrin and complex salen) and employing biological (endophytes). This will be developed and validated chromatographic methods (HPLC-MS,HPLC-MS/MS) for analyzing the products of metabolism. It is still part of the objectives of this project to obtain more active metabolites possibly preparative scale. Within this overall context this project aims to implement a work platform that will allow preclinical studies involving the metabolism of natural products groups included in the project "Development of a Platform for the Study of Metabolism In Vivo and In Vitro Products Natural System A Need for Pre-Clinical Trials. "

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, BRUNO A.; ASSIS, MARILDA D.; PETI, ANA P. F.; MORAES, LUIZ A. B.; MOREIRA, FERNANDA L.; LOPES, NORBERTO P.; POSPISIL, STANISLAV; GATES, PAUL J.; DE OLIVEIRA, ANDERSON R. M. In vitro metabolism of monensin A: microbial and human liver microsomes models. XENOBIOTICA, v. 44, n. 4, p. 326-335, APR 2014. Web of Science Citations: 8.

ROCHA, BRUNO ALVES; MORAES DE OLIVEIRA, ANDERSON RODRIGO; PAZIN, MURILO; DORTA, DANIEL JUNQUEIRA; RODRIGUES, ANDRESA PIACEZZI NASCIMENTO; BERRETTA, ANDRESA APARECIDA; PETI, ANA PAULA FERRANTI; BERALDO DE MORAES, LUIZ ALBERTO; LOPES, NORBERTO PEPORINE; POSPISIL, STANISLAV; GATES, PAUL JONATHAN; ASSIS, MARILDA DAS DORES. Jacobsen Catalyst as a Cytochrome P450 Biomimetic Model for the Metabolism of Monensin A. BIOMED RESEARCH INTERNATIONAL, 2014. Web of Science Citations: 2.

SOUSA-JUNIOR, JOSE N.; ROCHA, BRUNO A.; ASSIS, MARILDA D.; PETI, ANA P. F.; MORAES, LUIZ A. B.; IAMAMOTO, YASSUKO; GATES, PAUL J.; DE OLIVEIRA, ANDERSON R. M.; LOPES, NORBERTO P. Biomimetic oxidation studies of monensin A catalyzed by metalloporphyrins: identification of hydroxyl derivative product by electrospray tandem mass spectrometry. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 23, n. 4, p. 621-629, Ago. 2013. Web of Science Citations: 5.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROCHA, Bruno Alves. Evaluation of microbiological and chemical models for the study of (bio)transformations of the antibiotic monensin A. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Ribeirão Preto.