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Magnetic resonance evaluation in a rodent model of intracerebral hemorrhage

Grant number: 11/15163-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2011
Effective date (End): September 30, 2012
Field of knowledge:Health Sciences - Medicine
Principal researcher:João Pereira Leite
Grantee:Patrícia Approbato Marques
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Introduction: Stroke (CVA) is the leading cause of death in Brazil and the leading cause of disability worldwide. The hemorrhagic stroke accounts for 10 to 25% of cases, and has the worst prognosis, with up to 65% mortality at 1 year. Using a murine model of intracerebral hemorrhage allows the study of this pathology in vivo to better understand the development of real-time injury. Through the acquisition of magnetic resonance imaging using three-dimensional sequences to measure the volume of intracerebral lesions is possible to study the dynamics of cerebral hemorrhage and assess the outcomes of interventions in a noninvasive manner and without sacrificing the animal. The objective of this study is the use of various sequences of MRI to characterize the neuroimaging findings related to brain injury caused by the injection of autologous blood in a murine model of hemorrhagic stroke, and its correlation with morphological changes. Methods: Wistar rats, male, 60 to 270 days, will undergo surgery stereotactic microinjection of 130 ml of autologous blood in the left striatum through the double-injection method. Behavioral testing will take place Placing forelimb, forelimb use asymmetry, Open Field Test and Neurological Deficit Scale for the presence of contralateral motor deficit. The MRI procedure will be made for monitoring in vivo and structural volume of intracerebral lesions. Histological analysis using cresyl violet staining and Fluoro-Jade B will be made to analyze measures of brain areas, cell quantification and verification of neuronal death 14 days after induction of ICH.