Paper-based platforms for noninvasive detection of tumor biomarkers and cultive neoplastic cells

• Grant number: 11/13997-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2011
• End date: October 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Emanuel Carrilho
• Grantee: Thiago Mazzu Do Nascimento
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

This project aims to create a platform for immunochromatographic assays, which allows a noninvasive detection of tumor biomarkers (sarcosine, BTA, CA 15-3) testing samples of urine and saliva. Along with the immunochromatographic platform, it will be tested the cultivation technique of cells on paper to evaluate the growth profile of neoplastic cells and tumor aggressiveness. The plataform of immunochromatographic assay will receive wax patterns printed by a conventional printer, that creates hydrophobic limits on paper, forming of wax microchannels. For the wax to cross the entire thickness of the paper, there will be a heat treatment, where the increase in temperature will melt the wax, which will go to cross all the paper. After drying, the wax creates a hydrophobic barrier that delimits the microchannel where the reaction occurs. In each microchannel is detected a different biomarker. In each microchannel will be contained specific antibodies immobilized in a region called the test area, another region containing the immobilized biomarker protein in a control zone. Through conjugation of antibodies with colloidal gold nanoparticles will be held immunochromatographic detection of these analytesin the cell culture on paper, it will be used layers of paper with hydrogel containing nutrients to promote cell growth and a plastic mask to separate each microzone. Once created the platform we will add cells from neoplastic tissue and evaluated the potential of this technique to be applied in cell based assays. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THIAGO MAZZU-NASCIMENTO; DÉBORA GUSMÃO MELO; GIORGIO GIANINI MORBIOLI; EMANUEL CARRILHO; FERNANDA SALES LUIZ VIANNA; ANDRÉ ANJOS DA SILVA; LAVINIA SCHULER-FACCINI. Teratogens: a public health issue - a Brazilian overview. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 2, p. 387-397, Jun. 2017. (11/13997-8)

THIAGO MAZZU-NASCIMENTO; DÉBORA GUSMÃO MELO; GIORGIO GIANINI MORBIOLI; EMANUEL CARRILHO; FERNANDA SALES LUIZ VIANNA; ANDRÉ ANJOS DA SILVA; LAVINIA SCHULER-FACCINI. Teratogens: a public health issue – a Brazilian overview. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 2, p. 387-397, 2017-05-22. (11/13997-8)

THIAGO MAZZU-NASCIMENTO; FABIANA CRISTINA DONOFRIO; BEATRIZ CUTILAK BIANCHI; REGIANE DE FÁTIMA TRAVENSOLO; JOÃO BATISTA SOUZA JUNIOR; DANIEL ANGELI MORAES; GIORGIO GIANINI MORBIOLI; THIAGO PINOTTI SEGATO; LAUDEMIR CARLOS VARANDA; EMANUEL CARRILHO. Paper-Based Microfluidics Immunoassay for Detection of Canine Distemper Virus. Brazilian Archives of Biology and Technology, v. 60, 2018-01-08. (11/13997-8, 12/02093-3)

MAZZU-NASCIMENTO, THIAGO; GOMES CARNEIRO LEAO, PAULO AUGUSTO; CATAI, JONATAN RICARDO; MORBIOLI, GIORGIO GIANINI; CARRILHO, EMANUEL. Towards low-cost bioanalytical tools for sarcosine assays for cancer diagnostics. ANALYTICAL METHODS, v. 8, n. 40, p. 7312-7318, 2016. (11/13997-8, 08/57805-2)

MORBIOLI, GIORGIO GIANINI; MAZZU-NASCIMENTO, THIAGO; STOCKTON, AMANDA M.; CARRILHO, EMANUEL. Technical aspects and challenges of colorimetric detection with microfluidic paper-based analytical devices (mu PADs) - A review. Analytica Chimica Acta, v. 970, p. 1-22, JUN 1 2017. (11/13997-8, 08/57805-2)

MORBIOLI, GIORGIO GIANINI; MAZZU-NASCIMENTO, THIAGO; MILAN, LUIS APARECIDO; STOCKTON, AMANDA M.; CARRILHO, EMANUEL. Improving Sample Distribution Homogeneity in Three-Dimensional Microfluidic Paper-Based Analytical Devices by Rational Device Design. Analytical Chemistry, v. 89, n. 9, p. 4786-4792, MAY 2 2017. (11/13997-8, 08/57805-2)

MAZZU-NASCIMENTO, THIAGO; MORBIOLI, GIORGIO GIANINI; MILAN, LUIS APARECIDO; DONOFRIO, FABIANA CRISTINA; MESTRINER, CARLOS ALBERTO; CARRILHO, EMANUEL. Development and statistical assessment of a paper-based immunoassay for detection of tumor markers. Analytica Chimica Acta, v. 950, p. 156-161, JAN 15 2017. (11/13997-8, 08/57805-2)

MAZZU-NASCIMENTO, THIAGO; MELO, DEBORA GUSMAO; MORBIOLI, GIORGIO GIANINI; CARRILHO, EMANUEL; LUIZ VIANNA, FERNANDA SALES; DA SILVA, ANDRE ANJOS; SCHULER-FACCINI, LAVINIA. Teratogens: a public health issue - a Brazilian overview. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 2, p. 11-pg., 2017-04-01. (11/13997-8)

MORBIOLI, GIORGIO GIANINI; MAZZU-NASCIMENTO, THIAGO; AQUINO, ADRIANO; CERVANTES, CESAR; CARRILHO, EMANUEL. Recombinant drugs-on-a-chip: The usage of capillary electrophoresis and trends in miniaturized systems - A review. Analytica Chimica Acta, v. 935, p. 44-57, SEP 7 2016. (08/57805-2, 11/13997-8)

MAZZU-NASCIMENTO, THIAGO; MORBIOLI, GIORGIO GIANINI; MILAN, LUIS APARECIDO; SILVA, DIEGO FURTADO; DONOFRIO, FABIANA CRISTINA; MESTRINER, CARLOS ALBERTO; CARRILHO, EMANUEL. Improved assessment of accuracy and performance indicators in paper-based ELISA. ANALYTICAL METHODS, v. 9, n. 18, p. 2644-2653, MAY 14 2017. (11/13997-8, 08/57805-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NASCIMENTO, Thiago Mazzu Do. Low-cost paper-based platforms for immunodiagnostic and enzymatic testing. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.