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Effects of cats (FAM64A) depletion and overexpression on the proliferation of the leukemic cell line U937

Grant number: 11/18188-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2011
Effective date (End): June 30, 2013
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Leticia Fröhlich Archangelo
Grantee:Isabella Barbutti Gonçalves
Home Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The novel protein CATS (FAM64A) was recently described as interacting with both CALM and the leukemic fusion protein CALM/AF10. The interaction between CATS and CALM may be related to leukemogenesis caused by CALM/AF10. CATS is known to be associated to cell proliferation and cell division, more specificly controlling the metaphase-anaphase transition. CATS localizes in the nucleus and nucleoli, causing both CALM and CALM/AF10 to accumulate in those structures due to the CATS-CALM interaction. It has been suggested that CATS may participate in leukemogenesis because CATS can interact with CALM/AF10, influences its subcellular localization and is overexpressed in leukemic cells. The aim of this study is to investigate CATS function in the monocitic leukemia cell line U937, which has the fusion protein CALM/AF10. Therefore the lentiviral system will be used in order to produce virus that can induce CATS overexpression and depletion when transduced with U937 cell line. To confirm CATS overexpression and depletion qRT-PCR and Western blotting will be made. CATS overexpressed and CATS depleted cells will undergo fuctional assays, in order to evaluate cell proliferation and cell cycle progression.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBUTTI, ISABELLA; XAVIER-FERRUCIO, JULIANA M.; MACHADO-NETO, JOAO AGOSTINHO; RICON, LAUREMILIA; TRAINA, FABIOLA; BOHLANDER, STEFAN K.; OLALLA SAAD, SARA TERESINHA; ARCHANGELO, LETICIA FROHLICH. CATS (FAM64A) abnormal expression reduces clonogenicity of hematopoietic cells. ONCOTARGET, v. 7, n. 42, p. 68385-68396, OCT 18 2016. Web of Science Citations: 4.

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