Influence of lycopene supplementation on oxidative stress in diet-induced obesity model

• Grant number: 11/19738-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Ana Lucia do Anjos Ferreira
• Grantee: Paula Torres Presti
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Most of studies regarding obesity have been shown increase of oxidative event. This event results in deficiency of antioxidant defense and /or increase in reactive species (oxygen, nitrogen). However, few studies have been evaluated the effect of the important dietetic antioxidant lycopene on obesity and in diseases associate. The main aim this study is to evaluate the effect of chronic administration with lycopene on cardiac oxidative stress induced by hypercaloric diet. Male Wistar rats (n=28), ageing 60 days will be randomly separated for receiving control diet (C) or hypercaloric diet (HD) for 6 weeks. After this period, all animals will be again randomized into four groups: C (n=7), C supplemented with Lycopene (C+Ly, n=7), HC (n=7) or supplemented with HD and lycopene (HD+Ly, n=7). The lycopene used will be oil-resin mixed with corn oil at a dose of 10mg/Kg body weight/day and will be administered to animals via gavage for 6 weeks. During the same period the animals in untreated groups (C and HD) will receive corn oil via gavage at an equivalent volume offered to the groups treated with lycopene (~ 0.5mL/Kg/day). Food and water will be offered ad libitum, and the consumption will be daily recorded. Body weight will be weekly measured. Oxidative stress level will be determined by total antioxidant performance (TAP) and carbonylation of proteins. The arterial blood pressure and the blood of all the animals will be submitted to laboratorial evaluation (glucose, insulin, triglycerides and total cholesterol), lycopene concentration, antioxidant capacity (TAP) at sixth (M6), and twelfth (M12) week of the experiment. At 12 (M12), when the animals will be killed, it will be evaluated the total body fat, and also TAP and carbonylation in cardiac tissue (left ventricle). (AU)