Prognostic value of the genic and protein expression of the ATM in bitches with different mammary neoplasms

• Grant number: 10/13777-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Talita Mariana Morata Raposo Ferreira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Breast cancer is the main tumor that affects bitches and their biological behavior is similar to what occurs in women, therefore bitches can be an excellent model for comparative study of the carcinogenic process in this type of cancer. Metastasis is a common consequence and major cause of mortality. The identification of tumor markers can be a useful tool in the evaluation of prognostic and predictive factors. Thus, this study aims to evaluate the genic and protein expression of ATM and other markers such p53 and VEGF by RT-PCR and immunohistochemistry, respectively. p53 and VEGF are controlled by ATM and implicated in metastatic process. These markers seem to have an important interaction in women breast cancer, therefore the study of their association can provide a new perspective on the treatment and prognosis of bitches breast cancer. 40 canine mammary gland will be used divided in four groups: G1= normal mammary gland, no history of cancer, comprising 10 animals; G2= 10 benign tumor; G3 = 10 malignant mammary tumors without evidence of metastasis; G4= 10 bitches with malignant breast tumor and metastasis. The results will be evaluated for the presence of each marker in the tissues, its relationship with the biological behavior and histological type of the tumor, recurrence and/or metastasis by the method of analysis of variance. Thereby, we hoped to better understand the involvement of these markers in the carcinogenic process and development of metastasis and provide further data on the prognosis in bitches.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAPOSO-FERREIRA, T. M. M.; BUENO, R. C.; TERRA, E. M.; AVANTE, M. L.; TINUCCI-COSTA, M.; CARVALHO, M.; CASSALI, G. D.; LINDE, S. D.; ROGATTO, S. R.; LAUFER-AMORIM, R.. Downregulation of ATM Gene and Protein Expression in Canine Mammary Tumors. VETERINARY PATHOLOGY, v. 53, n. 6, p. 1154-1159, NOV 2016. (11/10279-7, 10/13777-5)

MORATA RAPOSO-FERREIRA, TALITA MARIANA; VARALLO, GIOVANNA ROSSI; CALAZANS, SABRYNA GOUVEIA; JARK, PAULO CESAR; LINO SALVADOR, ROSANA DA CRUZ; TINUCCI-COSTA, MIRELA; DE NARDI, ANDRIGO BARBOZA; CASSALI, GEOVANNI DANTAS; LAUFER-AMORIM, RENEE. Paraneoplastic Hypercalcemia Secondary to Canine Mammary Tumors. Acta Scientiae Veterinariae, v. 44, SEP 6 2016. (10/13777-5, 11/10279-7)

RAPOSO-FERREIRA, TALITA M. M.; SALVADOR, ROSANA C. L.; TERRA, ERIKA M.; FERREIRA, JUAREZ H.; VECHETTI-JUNIOR, IVAN JOSE; TINUCCI-COSTA, MIRELA; ROGATTO, SILVIA R.; LAUFER-AMORIM, RENEE. Evaluation of vascular endothelial growth factor gene and protein expression in canine metastatic mammary carcinomas. MICROSCOPY RESEARCH AND TECHNIQUE, v. 79, n. 11, p. 1097-1104, NOV 2016. (11/10279-7, 10/13777-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERREIRA, Talita Mariana Morata Raposo. Valor prognóstico da expressão gênica e proteica do ATM, p53 e VEGF em cadelas com neoplasias mamárias. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.