Characterization of an animal model for type 2 diabetes and obesity and their potential use for cell therapy

• Grant number: 11/05440-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2013
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Flavio Henrique Caetano
• Grantee: Adriane Paduan Nolli
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Type 2 diabetes mellitus (T2DM) occurs when insulin secretion is inadequate to maintain the normal concentration of glucose in the blood and is often associated with obesity related to insulin resistance and / or its failure, absolute or relative. In the long term, it is accompanied by dysfunction and failure of various organs, especially eyes, kidneys, nerves, heart and blood vessels. People with diabetes often show signs of accelerated atherosclerosis, suffer acute coronary syndromes, myocardial infarction with silent myocardial ischemia and peripheral arterial disease. These patients have severe impairments in endothelial cell progenitors, which participate in angiogenesis. This work will be a hybrid animal model recently described in 2009 by Karasawa et al., result from the crossing of inbred strains of mice DBA / 2 and C57BL / 6, resulting in the model BDF1 (F1 generation of breeding), which is considered a appropriate model for the study of type 2 diabetes associated with obesity. According to the authors Leiter et al. (1981) and Smith et al. (2000), the model could become BDF1 diabetic obese by intake of high fat diet. This model is poorly characterized with high potential for studies of obesity and T2DM, which despite its prevalence in the population is rarely studied by the scientific community due to its limitation of appropriate models that mimic various aspects of the reality of the disease. Porting, the general objective of this work will characterize the potential for using this model for cell therapy analyzing the profile of progenitor cells exist in bone marrow and peripheral blood of these animals, as well as the potential of these cells to migrate to regions of tissue injury.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOLLI, Adriane Paduan. Caracterização do modelo B6D2F1 para obesidade, diabetes e síndrome metabólica e sua potencialidade para terapia celular em lesão aguda. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.