Analysis of the effect of G-CSF in the neuritogenic cells migration and effector pattern during the clinical course of experimental autoimmune neuritis induced in Lewis rats

• Grant number: 11/15175-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: March 31, 2013
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Alessandro dos Santos Farias
• Grantee: Fernando Pradella
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The Experimental autoimmune neuritis (EAN) is considered the experimental model of Guillain-Barré's syndrome. The EAN is an autoimmune disease characterized by the infiltration of mononuclear cells into the endoneural space. There is an activation of auto-reactive cells against peripheral myelin compounds. This response in characterized mainly by lymphocytes Th1 and/or Th17, which culminate with the myelin destruction. During the scientific initiation program we were able to demonstrate that the treatment with one hematopoietic factor, G-CSF, ameliorates the clinical signs of EAN. The group treated with G-CSF decreased the expression of proinflammatory cytokines (IFNg, IL-17a) and increased the expression of anti-inflammatory cytokines (IL-10, TGFb) and the percentage of regulatory T cells. However, our data do not explain completely the phenomena. The use of lower dosis did not present the changes of inflammatory profile observed for the optimal dose. Despite of that, the lower dosis ameliorates the clinical signs and decrease the number of cells infiltrated into the nerve. Therefore, is our objective to study the migratory pattern, using adoptive transfer of GFP transduced neuritogenic cells, during the evolution of EAN in the treated and untreated animals.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, ADRIEL S.; PAULA, ROSEMEIRE F. O.; PRADELLA, FERNANDO; SANTOS, MARIANA P. A.; OLIVEIRA, ELAINE C.; VON GLEHN, FELIPE; CAMILO, DANIELA S.; CERAGIOLI, HELDER; PETERLEVITZ, ALFREDO; BARANAUSKAS, VITOR; et al. The Suppressive Effect of IL-27 on Encephalitogenic Th17 Cells Induced by Multiwalled Carbon Nanotubes Reduces the Severity of Experimental Autoimmune Encephalomyelitis. CNS Neuroscience & Therapeutics, v. 19, n. 9, p. 682-687, SEP 2013. (12/04565-0, 11/18728-5, 11/15175-5, 11/15639-1, 12/09879-2, 12/01408-0)

FARIAS, ALESSANDRO S.; SPAGNOL, GABRIELA S.; BORDEAUX-REGO, PEDRO; OLIVEIRA, CAMILA O. F.; FONTANA, ANA GABRIELA M.; DE PAULA, ROSEMEIRE F. O.; SANTOS, MARIANA P. A.; PRADELLA, FERNANDO; MORAES, ADRIEL S.; OLIVEIRA, ELAINE C.; et al. Vitamin D-3 Induces IDO+ Tolerogenic DCs and Enhances Treg, Reducing the Severity of EAE. CNS Neuroscience & Therapeutics, v. 19, n. 4, p. 269-277, APR 2013. (11/18728-5, 11/15175-5, 12/01408-0, 11/15639-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PRADELLA, Fernando. Effect of the administration of the G-CSF onto the effector and immuneregulatory mechanisms of the experimental autoimmune neuritis induced in Lewis rats. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.