Identification and characterization of new enzymes with potential for lignocellulosic biomass conversion

• Grant number: 11/21608-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2012
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Igor Polikarpov
• Grantee: Bruno Luan Soares Paula de Mello
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 12/07504-1 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery, BE.EP.DD

Abstract

The raising of the use of fuels, the oil's finite supply and concerns about global warming make it clear that the search for new fuels is important. One answer for this problem is the use of lignocellulosic biomass (agricultural waste, for example) - which make up about 75% of dry weight of plant cell walls. This process uses enzymatic hydrolysis, which is one of the most complex and expensive steps nowadays. While the saccharification of lignocellulose remains a problem for industry, it is carried out effectively in the natural environment by microbial communities. Because less than 1% of the microorganisms can grow in pure cultures, the cellulose hydrolysis have been studied in a few well characterized organisms. Thus, the discovery and study of new enzymes and proteins associated with the lignocellulose degradation is necessary for the development of a efficient and cost-effective process. This can be achieved by the use of an integrated proteomics and metatranscriptomics approach. Therefore, the objectives of this work are discover new enzymes related with lignocellulose hydrolysis, select the most promising ones and characterize their biochemistry and three-dimensional structure. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PUHL, ANA C.; PRATES, ERICA T.; ROSSETO, FLAVIO R.; MANZINE, LIVIA R.; STANKOVIC, IVANA; DE ARAUJO, SIMARA S.; ALVAREZ, THABATA M.; SQUINA, FABIO M.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern. International Journal of Biological Macromolecules, v. 136, p. 493-502, SEP 1 2019. (11/20505-4, 15/13684-0, 09/52840-7, 10/52362-5, 11/21608-1, 15/50590-4)

GODOY, ANDRE S.; PEREIRA, CAROLINE S.; RAMIA, MARINA PAGLIONE; SILVEIRA, RODRIGO L.; CAMILO, CESAR M.; KADOWAKI, MARCO A.; LANGE, LENE; BUSK, PETER K.; NASCIMENTO, ALESSANDRO S.; SKAF, MUNIR S.; et al. Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members. SCIENTIFIC REPORTS, v. 8, FEB 27 2018. (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)

MELLO, BRUNO L.; ALESSI, ANNA M.; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia. SCIENTIFIC REPORTS, v. 6, DEC 12 2016. (11/21608-1, 10/52362-5)

EVANGELISTA, DANILO ELTON; ARNOLDI PELLEGRIN, VANESSA DE OLIVEIRA; SANTO, MELISSA ESPIRITO; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-beta-xylanase identified in a microbial consortium. Applied Microbiology and Biotechnology, v. 103, n. 19, p. 8035-8049, OCT 2019. (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)

EVANGELISTA, DANILO ELTON; SEIKI KADOWAKI, MARCO ANTONIO; MELLO, BRUNO LUAN; POLIKARPOV, IGOR. Biochemical and biophysical characterization of novel GH10 xylanase prospected from a sugar cane bagasse compost-derived microbial consortia. International Journal of Biological Macromolecules, v. 109, p. 560-568, APR 2018. (11/20505-4, 11/21608-1, 10/52362-5, 15/13684-0)

MELLO, BRUNO L.; ALESSI, ANNA M.; RIANO-PACHON, DIEGO M.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. G.; ESPIRITO SANTO, MELISSA C.; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-beta-xylanase activity. BIOTECHNOLOGY FOR BIOFUELS, v. 10, NOV 2 2017. (11/21608-1, 10/52362-5, 15/13684-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MELLO, Bruno Luan Soares Paula de. Identification and characterization of the first exo-xylanase from glycosil hydrolase family 11 from the study of the metatranscriptome of a compost-derived consortia. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.