Transdentinal and direct effect of infrared LED on human dental pulp cells and pulp-dentin complex

• Grant number: 11/13895-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Josimeri Hebling Costa
• Grantee: Ana Paula Silveira Turrioni
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 13/17758-3 - Effect of the isolation technique, source of tissue and cell passage on the number and phenotype of stem cells originating from dental pulp, BE.EP.DR

Abstract

Several studies have demonstrated that phototherapy may increase dentin matrix synthesis and deposition by odontoblasts as well as decrease the local inflammatory reaction. The light source generated by light emitting diodes (LED) has been widely studied, and several positive effects in tissue repair have been reported. Therefore, the objective of this study will be to evaluate the effect of LED 850 nm on stem cells from dental pulp and dentin-pulp complex. For the laboratory experiments, stem cells from dental pulp will be seeded on acrylic plates and /or on 0.2-mm-thick dentin disks obtained from sound human molars (n = 102). The LED will be applied directly on cells and/or on the occlusal surface of the dentin discs. Cell metabolism (MTT), alkaline phosphatase expression (ALP), total protein synthesis, mineralized nodule formation (Alizarin Red), cell proliferation (Trypan Blue) and cell morphology (MEV) will be evaluated. The expression of genes that encode for collagen type-1 (Col-1), dentin sialoprotein (DSPP), dentin matrix protein (DMP-1), alkaline phosphatase (ALP) and octamer-binding transcription factor 4 (OCT-4) will be analyzed by RT-PCR. For in vivo study, Class I cavities (n = 150) will be prepared on the proximal surface of first molars of rats (n = 60), with the presence or absence of pulp exposure. The cavities will be irradiated with the best parameter of LED, determined by the laboratory experiments, and submitted to direct pulp capping with calcium hydroxide (for the group with pulp exposure) and /or restored with glass ionomer cement. After 7, 15 or 30 days, the animals will be sacrificed and teeth will be processed for histological and immunohistochemical analysis. Statistical tests will be selected depending on the type of variable and data set.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANA PAULA SILVEIRA TURRIONI; LIEGE ALDROVANDI MONTORO; FERNANDA GONÇALVES BASSO; LEOPOLDINA DE FÁTIMA DANTAS DE ALMEIDA; CARLOS ALBERTO DE SOUZA COSTA; JOSIMERI HEBLING. Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation. Brazilian Dental Journal, v. 26, n. 4, p. 409-415, 2015-08-00. (13/17758-3, 11/13895-0)

TURRIONI, ANA PAULA S.; BASSO, FERNANDA G.; MONTORO, LIEGE A.; DE ALMEIDA, LEOPOLDINA DE FATIMA D.; DE SOUZA COSTA, CARLOS A.; HEBLING, JOSIMERI. Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth. Journal of Dentistry, v. 42, n. 10, p. 1292-1299, OCT 2014. (11/13895-0, 13/17758-3)

TURRIONI, A. P. S.; BASSO, F. G.; ALONSO, J. R. L.; DE OLIVEIRA, C. F.; HEBLING, J.; BAGNATO, V. S.; DE SOUZA COSTA, C. A.. Transdentinal Cell Photobiomodulation Using Different Wavelengths. Operative Dentistry, v. 40, n. 1, p. 102-111, JAN-FEB 2015. (09/03615-0, 11/13895-0)

TURRIONI, ANA PAULA S.; ALONSO, JULIANA R. L.; BASSO, FERNANDA G.; MORIYAMA, LILIAN T.; HEBLING, JOSIMERI; BAGNATO, VANDERLEI S.; DE SOUZA COSTA, CARLOS A.. LED light attenuation through human dentin: A first step toward pulp photobiomodulation after cavity preparation. AMERICAN JOURNAL OF DENTISTRY, v. 26, n. 6, p. 319-323, DEC 2013. (11/13895-0)

TURRIONI, A. P.; BASSO, F. G.; MONTORO, L. A.; ALMEIDA, L. F. D.; DE SOUZA COSTA, C. A.; HEBLING, J.. Transdentinal photobiostimulation of stem cells from human exfoliated primary teeth. International Endodontic Journal, v. 50, n. 6, p. 549-559, JUN 2017. (11/13895-0, 13/17758-3)