The nonenzymatic glycation of proteins (AGEs) is a common factor to the pathophysiology of a number of disorders related to aging and diseases such as diabetes mellitus (DM). The generation of AGEs products is through the reactions of non-enzymatic glycation of extracellular matrix in the dermis and have been touted as one of the factors responsible for disability and loss of elasticity of the skin healing. The skin permeation of compounds anti-AGE is a major limitation in the therapeutic efficacy of compounds that are expected to reach deeper layers of skin. Reconstructed skin containing glycated dermal equivalent are Models of three-dimensional structures that mimic human skin generated in vitro and represent an efficient model for the study of cells and changes in the extracellular matrix caused for the process of aging and diabetes. The 3D model of reconstructed skin has metabolic characteristics, permeability and activity similar to that of the original skin, increasing its role in investigations of drug permeability, toxicity, irritation and differentiation of keratinocytes. A series of natural or synthetic inhibitors compounds of AGEs have been discovered and presented recently and may represent therapeutic innovation in the treatment of modifications caused by the formation and accumulation of AGEs in the skin. This study aims to assess the development of artificial skin glycated and subsequently evaluating the efficacy and toxicity of anti-glycation compounds such as aminoguanidine and carnosine on glycated reconstructed skin model. In perspective, this study will contribute to the development of new technology in vitro glycated artificial skin, which help the understanding of the biology of cell-extracellular matrix mimicking important pathophysiological processes such as aging and diabetes.
News published in Agência FAPESP Newsletter about the scholarship:
PEDROSA, TATIANA DO NASCIMENTO;
CATARINO, CAROLINA MOTTER;
PENNACCHI, PAULA COMUNE;
DE ASSIS, SILVIA ROMANO;
LOPES CONSOLARO, MARCIA EDILAINE;
DE MORAES BARROS, SILVIA BERLANGA;
MARIA-ENGLER, SILVYA STUCHI.
A new reconstructed human epidermis for in vitro skin irritation testing.
TOXICOLOGY IN VITRO,
Web of Science Citations: 6.