Advanced search
Start date
Betweenand

Development of artificial skin containing glycated dermal equivalent mimicking the pathophysiology of skin aging and diabetes

Grant number: 11/22812-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): February 26, 2016
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal Investigator:Silvya Stuchi Maria-Engler
Grantee:Paula Comune Pennacchi
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The nonenzymatic glycation of proteins (AGEs) is a common factor to the pathophysiology of a number of disorders related to aging and diseases such as diabetes mellitus (DM). The generation of AGEs products is through the reactions of non-enzymatic glycation of extracellular matrix in the dermis and have been touted as one of the factors responsible for disability and loss of elasticity of the skin healing. The skin permeation of compounds anti-AGE is a major limitation in the therapeutic efficacy of compounds that are expected to reach deeper layers of skin. Reconstructed skin containing glycated dermal equivalent are Models of three-dimensional structures that mimic human skin generated in vitro and represent an efficient model for the study of cells and changes in the extracellular matrix caused for the process of aging and diabetes. The 3D model of reconstructed skin has metabolic characteristics, permeability and activity similar to that of the original skin, increasing its role in investigations of drug permeability, toxicity, irritation and differentiation of keratinocytes. A series of natural or synthetic inhibitors compounds of AGEs have been discovered and presented recently and may represent therapeutic innovation in the treatment of modifications caused by the formation and accumulation of AGEs in the skin. This study aims to assess the development of artificial skin glycated and subsequently evaluating the efficacy and toxicity of anti-glycation compounds such as aminoguanidine and carnosine on glycated reconstructed skin model. In perspective, this study will contribute to the development of new technology in vitro glycated artificial skin, which help the understanding of the biology of cell-extracellular matrix mimicking important pathophysiological processes such as aging and diabetes.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CATARINO, CAROLINA MOTTER; PEDROSA, TATIANA DO NASCIMENTO; PENNACCHI, PAULA COMUNE; DE ASSIS, SILVIA ROMANO; GIMENES, FABRICIA; LOPES CONSOLARO, MARCIA EDILAINE; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 125, p. 51-57, APR 2018. Web of Science Citations: 1.
PEDROSA, TATIANA DO NASCIMENTO; CATARINO, CAROLINA MOTTER; PENNACCHI, PAULA COMUNE; DE ASSIS, SILVIA ROMANO; GIMENES, FABRICIA; LOPES CONSOLARO, MARCIA EDILAINE; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. A new reconstructed human epidermis for in vitro skin irritation testing. TOXICOLOGY IN VITRO, v. 42, p. 31-37, AUG 2017. Web of Science Citations: 6.
IMMICH, A. P. S.; PENNACCHI, P. C.; NAVES, A. F.; FELISBINO, S. L.; BOEMO, R. L.; MARIA-ENGLER, S. S.; CATALANI, L. H. Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 48-58, APR 1 2017. Web of Science Citations: 4.
PENNACCHI, PAULA COMUNE; SOARES DE ALMEIDA, MAIRA ESTANISLAU; ALVES GOMES, OCTAVIO LUIS; FAIAO-FLORES, FERNANDA; DE ARAUJO CREPALDI, MARIA CLARA; DOS SANTOS, MARINILCE FAGUNDES; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. Glycated Reconstructed Human Skin as a Platform to Study the Pathogenesis of Skin Aging. TISSUE ENGINEERING PART A, v. 21, n. 17-18, p. 2417-2425, SEP 1 2015. Web of Science Citations: 16.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.