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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft

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Immich, A. P. S. ; Pennacchi, P. C. ; Naves, A. F. ; Felisbino, S. L. ; Boemo, R. L. ; Maria-Engler, S. S. ; Catalani, L. H.
Total Authors: 7
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 73, p. 48-58, APR 1 2017.
Web of Science Citations: 4

Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (L-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30 days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/18017-1 - Development of electrospun polymeric matrices FOR “The regeneration OF tympanic membrane perforations
Grantee:Ana Paula Serafini Immich Boemo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/22812-1 - Development of artificial skin containing glycated dermal equivalent mimicking “The pathophysiology OF skin aging ánd diabetes
Grantee:Paula Comune Pennacchi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/21442-6 - Synthetic and natural polymers applied to tissue engineering
Grantee:Luiz Henrique Catalani
Support type: Research Projects - Thematic Grants