Tympanic membrane perforations are due to common otological problems, usually resulting from otitis media or trauma. Audiological manifestations of tympanic perforations include hearing loss, buzzing and otorrhea. Perforation treatment intends to heal the tympanic tissue in order to prevent infections and restore hearing. One of the treatments includes perforations repairing through new polymeric matrices which provide a support for tissue regeneration (myringoplasty). The main objectives of this study are: development and characterization of electrospun polymeric membranes obtained from bioreabsorbable polymers, such as, poly-L-lactide (PLLA), poly(lactic-co-glycolic acid) (PLGA), policaprolactone (PCL), polyadipate (PIA) and poly(isosorbide-co-L-lactic succinate) (PIS-PLLA); and employment of these membranes as scaffolds for cellular growth, in vitro model, through growth factor (GF) applications (fibroblast and epithelial) for the regeneration of perforated tympanic membranes, as well as verification in vivo of regeneration scaffolds with the best performance. For membrane characterization it will be carried out structural, thermal and mechanical property analyzes. Membranes with cell culture, from the in vitro model, will be lead to MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to test cell viability and proliferation. The in vivo study, in mice, will be performed in two surgical procedures: bilateral myringotomy and myringoplasty of the right ear. To evaluate tissue regenerative results, histological analyzes will be carried out and the data will be treated through variance analyzes with global significance level of 95%.
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