DEVELOPMENT OF ELECTROSPUN POLYMERIC MATRICES FOR THE REGENERATION OF TYMPANIC MEMBRANE PERFORATIONS.

• Grant number: 11/18017-1
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2012
• Effective date (End): August 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Henrique Catalani
• Grantee: Ana Paula Serafini Immich Boemo
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Tympanic membrane perforations are due to common otological problems, usually resulting from otitis media or trauma. Audiological manifestations of tympanic perforations include hearing loss, buzzing and otorrhea. Perforation treatment intends to heal the tympanic tissue in order to prevent infections and restore hearing. One of the treatments includes perforations repairing through new polymeric matrices which provide a support for tissue regeneration (myringoplasty). The main objectives of this study are: development and characterization of electrospun polymeric membranes obtained from bioreabsorbable polymers, such as, poly-L-lactide (PLLA), poly(lactic-co-glycolic acid) (PLGA), policaprolactone (PCL), polyadipate (PIA) and poly(isosorbide-co-L-lactic succinate) (PIS-PLLA); and employment of these membranes as scaffolds for cellular growth, in vitro model, through growth factor (GF) applications (fibroblast and epithelial) for the regeneration of perforated tympanic membranes, as well as verification in vivo of regeneration scaffolds with the best performance. For membrane characterization it will be carried out structural, thermal and mechanical property analyzes. Membranes with cell culture, from the in vitro model, will be lead to MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to test cell viability and proliferation. The in vivo study, in mice, will be performed in two surgical procedures: bilateral myringotomy and myringoplasty of the right ear. To evaluate tissue regenerative results, histological analyzes will be carried out and the data will be treated through variance analyzes with global significance level of 95%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAVES, ALLINY F.; FERNANDES, HENRIQUE T. C.; IMMICH, ANA P. S.; CATALANI, LUIZ H.. Enzymatic syntheses of unsaturated polyesters based on isosorbide and isomannide. JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, v. 51, n. 18, p. 3881-3891, SEP 15 2013. (05/02855-7, 11/21442-6, 08/06445-6, 11/18017-1)

IMMICH, A. P. S.; PENNACCHI, P. C.; NAVES, A. F.; FELISBINO, S. L.; BOEMO, R. L.; MARIA-ENGLER, S. S.; CATALANI, L. H.. Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 48-58, APR 1 2017. (11/22812-1, 11/21442-6, 11/18017-1)