Zinc effect on the growth of mandibular condylar process of diabetic Wistar rats

• Grant number: 11/20185-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2012
• End date: February 28, 2013
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Edson Aparecido Liberti
• Grantee: Natália Santos de Camargo
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The cartilage of the condyle (PC) of the mandible of Wistar rats, the secondary type, presents an intense metabolic activity to maintain its integrity against chewing action. Insulin, an anabolic hormone essential in cartilage shows intense activity in the regulation of proliferation of chondrocytes. Diabetes mellitus (DM) is a group of metabolic disorders characterized by chronic hyperglycemia due to defects in secretion and/or insulin action, diretamete is related to zinc deficiency. Thus the objective of this study is noted in diabetic Wistar rats, chemically induced by streptozotocin and supplemented with zinc, the PC under the morphological and metabolic aspects. Will be obtained for both groups of animals (n = 5), diabetic (D), diabetic supplemented with 150 mg of zinc (DS150), and its counterpart represented by the control group (C). After euthanasia, histological processing and collection of biological samples, cellular components, and collagen will be evaluated as well as the tissue distribution of insulin receptors and intracellular (b), their detection by immunohistochemistry followed by quantification of reactive cells. The parameters quantified as the number of cells immunostained for each substance will be analyzed statistically.(AU)