Introduction: There are several reports that investigated the relationship between Diabetes mellitus (DM) to the severity periodontitis. These investigations showed the contribution of DM to severity of periodontitis via its effects on vasculature, inflammatory and immune response, alteration in collagen synthesis and genetic predisposition. The increased concentration of vascular endothelial growth factor (VEGF) has been considered an important factor in the pathogenesis of the chronic and aggressive periodontitis. Multiple lines of evidence suggested that Green tea consumption is associated with reduced risk of several human malignancies such as cancer and diabetes due to antiangiogenic effects. The green tea polyphenois with the epigallocatechin may exert inhibition the activation of the VEGF/VEGFR axis through suppressing the expression of HIF and others growth factors. This study investigated de relationship between VEGF, inflammation and periodontal disease in DM and the possible therapeutic effects of green tea in the modulation of angiogenesis.Materials and Methods: Serial sections (4µm thickness) will be obtained from the hemi-mandibles of streptozotocin-induced diabetic rat that ingested or not green tea (Camellia Sinensis) daily for 15, 30, 60 and 90 days (n=5 animals/group). In mesial root of first molar stained with hematoxylin-eosin will be evaluated the inflammatory process and alveolar bone loss. In the tissue sections obtained on silanized slides immunostained against VEGF and CD34 are evaluated, respectively, the percentage of VEGF-positive cells per mm ² of periodontal tissue and microvessel density (MD). Numerical data will be compared between groups using analysis of variance (ANOVA) and Tukey's post hoc test at significance level of 5%. The Pearson correlation coefficient will be carried to examine the degree relationship as well as direction sign (positive or negative) between the inflammatory infiltrate and alveolar bone loss variables versus the VEGF expression and MD.
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