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Immunohistochemical analysis of VEGF protein and microvessel density in the periodontium of diabetic rats treated with green tea.

Grant number: 12/00680-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Biological Sciences - Morphology - Histology
Principal researcher:Gerson Francisco de Assis
Grantee:Daniela Santos Pereira
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Introduction: There are several reports that investigated the relationship between Diabetes mellitus (DM) to the severity periodontitis. These investigations showed the contribution of DM to severity of periodontitis via its effects on vasculature, inflammatory and immune response, alteration in collagen synthesis and genetic predisposition. The increased concentration of vascular endothelial growth factor (VEGF) has been considered an important factor in the pathogenesis of the chronic and aggressive periodontitis. Multiple lines of evidence suggested that Green tea consumption is associated with reduced risk of several human malignancies such as cancer and diabetes due to antiangiogenic effects. The green tea polyphenois with the epigallocatechin may exert inhibition the activation of the VEGF/VEGFR axis through suppressing the expression of HIF and others growth factors. This study investigated de relationship between VEGF, inflammation and periodontal disease in DM and the possible therapeutic effects of green tea in the modulation of angiogenesis.Materials and Methods: Serial sections (4µm thickness) will be obtained from the hemi-mandibles of streptozotocin-induced diabetic rat that ingested or not green tea (Camellia Sinensis) daily for 15, 30, 60 and 90 days (n=5 animals/group). In mesial root of first molar stained with hematoxylin-eosin will be evaluated the inflammatory process and alveolar bone loss. In the tissue sections obtained on silanized slides immunostained against VEGF and CD34 are evaluated, respectively, the percentage of VEGF-positive cells per mm ² of periodontal tissue and microvessel density (MD). Numerical data will be compared between groups using analysis of variance (ANOVA) and Tukey's post hoc test at significance level of 5%. The Pearson correlation coefficient will be carried to examine the degree relationship as well as direction sign (positive or negative) between the inflammatory infiltrate and alveolar bone loss variables versus the VEGF expression and MD.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CATANZARO, DANIELA PEREIRA; MENA LAURA, EVER ELIAS; CESTARI, TANIA MARY; NUNES ARANTES, RICARDO VINICIUS; GARLET, GUSTAVO POMPERMAIER; TAGA, RUMIO; ASSIS, GERSON FRANCISCO. Green tea prevents vascular disturbs and attenuates periodontal breakdown in long-term hyperglycaemia in T1D rats. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 45, n. 5, p. 557-569, MAY 2018. Web of Science Citations: 1.

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