Generation of canine pluripotent stem cell through in vivo and in vitro mechanisms

• Grant number: 11/22915-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2012
• End date: June 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Natalia Juliana Nardelli Gonçalves
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated scholarship(s): 12/09631-0 - The establishment and culture of iPS from canine fibroblasts, BE.EP.DR

Abstract

Recent trials with animal models have shown the great importance of embryonic pluripotent stem cells for the treatment of various diseases, many of them extremely complex. Regenerative medicine supported by the development of biotechnology emerges with a promising future, one of the most desired therapeutic options. In this context, obtaining stem cell lineages from dog embryos is still a challenge as a model for cell therapy, however, some researchers have shown be possible to isolate these cells from inseminated and castrated females, even though a laborious process. In the other hand, the production of induced pluripotent stem cells (iPS) from dog fibroblasts reached encouraging results for obtaining pluripotent cells and the study of its application in regenerative medicine without severe ethical implications. This proposal is aimed to isolate and cultivate the dog embryo stem cells derived from pre implanted blastocysts, to establish a genetically induced pluripotent stem cells lineage from dogs (iPS - induced pluripotent stem cell), and subsequent characterization of them. It is expected, based on comparative analysis, the iPS produced (in vitro model) require the same conditions of maintenance, and exhibit the same growing behavior as common embryonic stem cells (in vivo model), confirming its applicability from a comparative model of development.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, N. J. N.; BRESSAN, F. F.; ROBALLO, K. C. S.; MEIRELLES, F. V.; XAVIER, P. L. P.; FUKUMASU, H.; WILLIAMS, C.; BREEN, M.; KOH, S.; SPER, R.; et al. Generation of LIF-independent induced pluripotent stem cells from canine fetal fibroblasts. Theriogenology, v. 92, p. 75-82, APR 1 2017. (15/09575-1, 12/09631-0, 11/22915-5)

GONCALVES, N. J. N.; BRESSAN, F. F.; SOUZA, A.; MARTINS, D. S.; MIGLINO, M. A.; MEIRELLES, F. V.; PERECIN, F.; AMBROSIO, C. E.. Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 47, p. 4-pg., 2012-12-01. (11/22915-5, 09/11631-6, 12/01060-4)

GONCALVES, N. J. N.; BRESSAN, F. F.; SOUZA, A.; MARTINS, D. S.; MIGLINO, M. A.; MEIRELLES, F. V.; PERECIN, F.; AMBROSIO, C. E.. Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 47, n. 6, SI, p. 84-87, DEC 2012. (09/11631-6, 12/01060-4, 11/22915-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GONÇALVES, Natalia Juliana Nardelli. Generation of canine pluripotent stem cells: factors involved in the establishment of reprogramming by gene induction. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.