PRODUCTION OF RECOMBINANT PROTEINS IN BHK-21 CELLS CULTURED IN SERUM-FREE MEDIUM

• Grant number: 12/00978-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2012
• End date: February 29, 2016
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Soraia Attie Calil Jorge
• Grantee: Sandra Fernanda Suárez Patiño
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The increased demand for recombinat proteins has been addressed the development of new technologies to improve production of safe and efficient biopharmaceuticals from the animal cell cultures. The culture medium used for the maintenance of these systems does not provide the features necessary for cells to grow on plastic culture substrates without the presence of fetal calf serum. However, the use of FCS is not advisable due to the variations found among the lots, the high content of animal protein and the possibility of the presence of infectious agents. For this reason, technological advances led to development of serum free medium, standardize and able to supply the needs of animal cells. Nevertheless, the process of adaptation of cells may be could take considerable time. Thus, this study aims to evaluate the metabolism of cells adapted to serum free medium and gene expression through the use of the Semliki Forest Virus-SFV system (RNA loading). Two genes have been chosen as a target for this study: the rabies virus glycoprotein (RVGP) and nonstructural protein 3 (NS3) of hepatitis C virus (HCV).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUAREZ-PATINO, SANDRA FERNANDA; BERNARDINO, THAISSA CONSONI; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; ASTRAY, RENATO MANCINI; PEREIRA, CARLOS AUGUSTO; SOARES, HUGO R.; COROADINHA, ANA S.; CALIL JORGE, SORAIA ATTIE. Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins. Cytotechnology, v. 71, n. 5, p. 949-962, OCT 2019. (11/05807-4, 12/00978-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PATIÑO, Sandra Fernanda Suárez. Production of recombinant proteins in BHK-21 cells cultured in serum free media.. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.