Hyperthermic and photodynamic therapies are well characterized as effective ways to eliminate tumors, but the interest now lays in the application of both therapies together. We aim to investigate the viability of hyperthermic therapy through gold nanorods activation (with laser at 810 nm) applied together with photodynamic therapy through Photogem® activation (with laser at 630 nm), either simultaneously or sequentially, as well as to synthesize gold nanorods conjugated with porphyrins and test their efficacy. Our nanorods will be synthesized by "seed-mediated" method, and both therapies will be applied on animals bearing Ehrlich adenocarcinoma. The pharmacokinetics of both photosensitizer and conjugated nanoparticles will be assessed by fluorescence spectroscopy, and the biochemical damage by tert-butil initiated chemiluminescence assay (which assesses membrane lipoperoxidation) and TRAP assay (which assesses the total antioxidant capacity of cells). Histological damage on tumor mass will be observed through optic microscopy with sections stained with hematoxylin-eosin. Besides, the animals will be observed long term to assess the survival rate after the treatments. Chemiluminescence curves will be analyzed by 2-way ANOVA, with Bonferroni as a post test, while the other results will be analyzed by 1-way ANOVA (results will be considered significant when p value is less than 0,05).
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