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Synergic action of photodynamic and hyperthermic therapies under different therapeutic application conditions.

Grant number: 11/23660-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): December 31, 2015
Field of knowledge:Engineering - Biomedical Engineering - Bioengineering
Principal researcher:Ana Maria de Guzzi Plepis
Grantee:Lucas Freitas de Freitas
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated scholarship(s):13/20599-4 - Synthesis, characterization and phototoxicity evaluation of gold nanorod-phthalocyanine complexes, BE.EP.DR


Hyperthermic and photodynamic therapies are well characterized as effective ways to eliminate tumors, but the interest now lays in the application of both therapies together. We aim to investigate the viability of hyperthermic therapy through gold nanorods activation (with laser at 810 nm) applied together with photodynamic therapy through Photogem® activation (with laser at 630 nm), either simultaneously or sequentially, as well as to synthesize gold nanorods conjugated with porphyrins and test their efficacy. Our nanorods will be synthesized by "seed-mediated" method, and both therapies will be applied on animals bearing Ehrlich adenocarcinoma. The pharmacokinetics of both photosensitizer and conjugated nanoparticles will be assessed by fluorescence spectroscopy, and the biochemical damage by tert-butil initiated chemiluminescence assay (which assesses membrane lipoperoxidation) and TRAP assay (which assesses the total antioxidant capacity of cells). Histological damage on tumor mass will be observed through optic microscopy with sections stained with hematoxylin-eosin. Besides, the animals will be observed long term to assess the survival rate after the treatments. Chemiluminescence curves will be analyzed by 2-way ANOVA, with Bonferroni as a post test, while the other results will be analyzed by 1-way ANOVA (results will be considered significant when p value is less than 0,05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREITAS, L. F.; HAMBLIN, M. R.; ANZENGRUBER, F.; PERUSSI, J. R.; RIBEIRO, A. O.; MARTINS, V. C. A.; PLEPIS, A. M. G.. Zinc phthalocyanines attached to gold nanorods for simultaneous hyperthermic and photodynamic therapies against melanoma in vitro. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 173, p. 181-186, . (11/23660-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FREITAS, Lucas Freitas de. Synergic action between photodynamic therapy and hyperthermic therapy using gold nanorods. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.

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