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Evaluation of the transcriptome and proteome of breast cancer cells with different expression profile of SPARC (secreted protein acidic and rich in cysteine ) in the presence and absence of docetaxel

Grant number: 12/03920-0
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): September 30, 2013
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Maria Aparecida Nagai
Grantee:Ana Carolina Pavanelli
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Breast cancer is a common disease with high morbidity and mortality. The etiology of breast cancer is heterogeneous and complex and this neoplastic is considered a multifactorial disease. Genetic predisposition is the major risk factor for breast cancer. The genetic alterations include activation of oncogenes and loss of function of cancer suppressor genes.Studies in our laboratory using the technique SAGE (Serial Analysis of Gene Expression) evaluated the gene expression profile in breast cancer cells that had different levels expression of HER2. Several genes were identified as differentially expressed, including SPARC, a matricelular protein as a potential marker of sensitivity to docetaxel chemotherapy.SPARC, because their participation in the extracellular matrix, influences many biological processes wich alter cell physiology and in cancer cases SPARC is widely involved in angiogenesis and tumor progression. The ambiguity of the behavior of SPARC expression in various tumor types suggests that SPARC overexpression correlates with disease progression and worse prognosis in other tumor types and its high expression has the function of tumor suppressor.In breast cancer studies suggest that overexpression of SPARC is associated with tumor progression, but other studies suggest low expression of SPARC worse prognosis and aggressive phenotype.In this study we intend to compare the transcriptome and proteome of breast cancer cells with and without expression of SPARC before and after treatment with docetaxel, for the characterization of gene and protein networks associated with the expression of SPARC protein and that may be involved in chemosensitivity to docetaxel.

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