Influence of genetic variants of eNOS in pacients with sistemic Hypertention

• Grant number: 12/02677-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2012
• Effective date (End): November 30, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Dorotéia Rossi Silva Souza
• Grantee: Sâmia Frahia Bento da Silva
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Systemic arterial Hypertension is recognized as an important factor for cardiovascular disease and has a significant prevalence in the global population. Recent literature has shown a relationship between hypertension and changes in nitric oxide (NO) metabolism. NO is synthesized from L-arginine, a constituent of the enzyme nitric oxide synthase (NOS). The eNOS isoform converts L-argenina into nitric oxide, culminating in vasodilation, regulation of vasomotor tonus, and blood pressure control in normotensive and hypertensive individuals. G894T eNOS polymorphism with a recognized relationship in the synthesis of NO and consequent changes in coronary vasomotor response is identified as a potential factor for the development of hypertension. This study aims to evaluate the association between the eNOS-G894T polymorphism and blood pressure change - in hypertensive and prehypertensive patients and characterize the presence or absence of metabolic disorders, dyslipidemia, and diabetes mellitus, often linked to hypertension. It will be selected 300 subjects, 100 controls, 100 hypertensives, and 100 pre-hypertensive. The eNOS G894T variants are to be analyzed by polymerase chain reaction (PCR) standard. The product after PCR is subjected to the RFLP (restriction fragment length polymorphism) with the restriction enzyme BanII ® (Fermentas) at 37 ° C for 16 hours, to identify the normal genotype, and with the enzyme MboI ® (Fermentas) at 37 ° C for 16 hours, to identify the mutation after enzymatic digestion product will be subjected to 1.5% agarose gel stained with GelRed ®. Statistical analysis will include the Fisher exact test and multivariate regression with a significance level P <0.05.(AU)