Saliva is an important protective factor for oral diseases due to their properties such as buffering capacity, formation of acquired pellicle and remineralizing enamel by means of Ca and P. However, many diseases can affect the production and composition of saliva, resulting in xerostomia. Estudos show that the main cause of dry mouth is radiation therapy in head and neck used to treat cancer. This is due to reduced salivary flow, and biochemical changes in the composition of saliva. The pediatric cancer is highly prevalent and important among them is the second most common lymphoma. However, it is not known from previous studies that have researched on xerostomia in children with lymphoma. Therefore, the objective of this study will evaluate the following properties of saliva: salivary flow, pH, buffering capacity, mineral composition (Ca, Pi) and total protein in pediatric patients diagnosed with lymphoma and who are under radiotherapy. There will be collection of stimulated saliva of 15 children aged 10 to 15 years, suffering from lymphoma Institute Infantil Boldrini. Will be used as a control 15 healthy children. Due to the volume of saliva collected in five minutes, will be estimated salivary flow in mL / min. The saliva collected will be used to evaluate the pH and buffering capacity, by colorimetric testing and determining the mineral content by atomic absorption spectrophotometry. Will determine the amount of total protein by the Lowry method, the activity of salivary amylase and electrophoresis to identify the proteins present. Will apply a statistical test adjusting in order to compare children with cancer with the control group.
News published in Agência FAPESP Newsletter about the scholarship: