This research has the following objectives: 1- To determine the concentration and activity of the isoenzyme CA VI in saliva and dental plaque and salivary flow, pH and buffering capacity of saliva and dental plaque of children with caries; 2 - Investigate the relationship between these variables and dental caries. For this study, 100 children aging 7 to 9 years will be divided into 02 groups: caries free group (Group LC n = 50) and caries group (Group C, n = 50). Chidren will be submitted to clinical examination, collection of saliva and plaque for analysis of salivary flow and concentration and activity of AC VI, analysis of pH and buffering capacity. For testing the concentration of salivary CA VI, we will use procedures for labeling and fluoroimmunoassay antigen. The analysis of the AC VI activity will be held by zymography. The determination of the concentration of the AC VI biofilm will be performed by immunohistochemistry. Salivary flow will be expressed in mL/min. The saliva pH will be measured with a commercial kit and of the biofilm with ion-selective electrode. The buffering capacity of saliva will be measured eletrometricaly. The correlations between the decay and the variables concentration and activity of AC VI isoenzyme, salivary flow, pH and buffering capacity of saliva and plaque will be assessed by Spearman correlation test. The bivariate analysis (chi-square test) will be held initially with the aim of studying possible associations between the dependent and independent variables. In multivariate modeling, associations between the dependent and independent variables are expressed as odds ratios (OR) with their respective confidence intervals of 95%.
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