Development of mucoadhesive semisolid systems for anesthetic release and iontophoresis application on the buccal cavity

• Grant number: 12/05644-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2012
• End date: January 31, 2014
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Renata Fonseca Vianna Lopez
• Grantee: Camila Cubayachi
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Local anesthesia of the oral cavity is a critical step for most dental procedures. The administration of anesthetics is usually done invasively, through injections that often scare away patients of the clinics. The effective non-invasive administration of local anesthetics in the oral cavity in order to replace the painful injections would enable the improvement of routine and surgical procedures because of the likely cost savings, patient compliance, ease of application and less risk of contamination and toxicity. However, the deep anesthesia of strategic areas of the oral mucosa during appropriate time requires penetration of the anesthetic in effective amounts. This high localized penetration is not achieved easily with the simple application of conventional semi-solid formulations. The rate of release and permeation of the drug may, however, be increased and modulated by using suitable pharmaceutical adjuvants and the application of iontophoresis. Thus, this project aims to develop mucoadhesive semi-solid systems for topical / local anesthetics release in the oral cavity, enabling the application of iontophoresis to direct and control their release. For this purpose, the formulations developed will be characterized physically and physico-chemically and in vitro and ex vivo tests will be undertaken to assess whether they are capable of being administrated in the oral cavity. Concentration, pH and ionic strength of the systems will be evaluated in order to increase the efficiency of electric current when applied to iontophoresis and thereby enabling the development of a suitable iontophoretic delivery system. Statistical tools will be used for the selection of optimized formulations with improved characteristics.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO COUTO, RENE OLIVEIRA; THOMAZ, DOUGLAS VIEIRA; DUARTE, MAIRA PEREZ FERREIRA; LOPEZ, RENATA FONSECA VIANNA; PEDRAZZI, VINICIUS; DE FREITAS, OSVALDO; TARTAGLIA, GIANLUCA MARTINO. Assessing α-Bisabolol as a Transmucosal Permeation Enhancer of Buccal Local Anesthetics. PHARMACEUTICS, v. 16, n. 9, p. 13-pg., 2024-09-01. (12/05644-0, 14/22451-7, 13/12174-3)

COUTO, RENE O.; CUBAYACHI, CAMILA; LOPEZ, RENATA F. V.; DE GAITANI, CRISTIANE M.; PEDRAZZI, VINICIUS; DE FREITAS, OSVALDO. A simple and high-resolution HPLC-PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies. BIOMEDICAL CHROMATOGRAPHY, v. 30, n. 6, p. 857-866, JUN 2016. (12/06167-1, 12/07251-6, 12/05644-0)

CUBAYACHI, CAMILA; DO COUTO, RENE OLIVEIRA; DE GAITANI, CRISTIANE MASETTO; PEDRAZZI, VINICIUS; DE FREITAS, OSVALDO; VIANNA LOPEZ, RENATA FONSECA. Needle-free buccal anesthesia using iontophoresis and amino amide salts combined in a mucoadhesive formulation. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 136, p. 1193-1201, DEC 1 2015. (13/12174-3, 12/05644-0)

DO COUTO, RENE OLIVEIRA; CUBAYACHI, CAMILA; CALEFI, PAULO LINARES; VIANNA LOPEZ, RENATA FONSECA; PEDRAZZI, VINICIUS; DE GAITANI, CRISTIANE MASETTO; DE FREITAS, OSVALDO. Combining amino amide salts in mucoadhesive films enhances needle-free buccal anesthesia in adults. JOURNAL OF CONTROLLED RELEASE, v. 266, p. 205-215, NOV 28 2017. (12/07251-6, 12/05644-0, 13/12174-3, 14/22451-7, 12/06167-1)

DO COUTO, RENE OLIVEIRA; CUBAYACHI, CAMILA; DUARTE, MAIRA PERES FERREIRA; LOPEZ, RENATA FONSECA VIANNA; PEDRAZZI, VINICIUS; GAITANI, CRISTIANE MASETTO DE; FREITAS, OSVALDO DE. Towards the advance of a novel iontophoretic patch for needle-free buccal anesthesia. Materials Science & Engineering C-Materials for Biological Applications, v. 122, MAR 2021. (12/05644-0, 14/22451-7, 13/12174-3)

SILVA FAVACHO, HUGO ALEXANDRE; DO COUTO, RENE OLIVEIRA; FERREIRA DUARTE, MAIRA PERES; GAROFO PEIXOTO, MARIA PAULA; VIANNA LOPEZ, RENATA FONSECA; PEDRAZZI, VINICIUS; DE GAITANI, CRISTIANE MASETTO; DE FREITAS, OSVALDO. Synergy between surfactants and mucoadhesive polymers enhances the transbuccal permeation of local anesthetics from freeze-dried tablets. Materials Science & Engineering C-Materials for Biological Applications, v. 108, MAR 2020. (12/05644-0, 12/06167-1, 12/07251-6, 13/12174-3, 14/22451-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CUBAYACHI, Camila. Development of mucoadhesive semisolid systems for anesthetic release and iontophoresis application on the buccal cavity. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.