Function of perilipin-5 on mitochondrial function and insulin resistance in skeletal muscle of wistar rats submitted to high-fat diet

• Grant number: 12/01265-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: September 01, 2012
• End date: April 30, 2013
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Sandro Massao Hirabara
• Grantee: Amanda Roque Martins
• Supervisor: Patrick Schrauwen
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Maastricht University, Maastricht (UM), Netherlands
• Associated to the scholarship: 10/05669-8 - Effect of supplementation with omega-3 polyunsaturated fatty acids and regular physical exercise on insulin resistance induced by high-fat diet, BP.DD

Abstract

There are several situations in which insulin resistance is common, such as obesity and type 2 Diabetes mellitus. In these situations it is also observed increased free fatty acid (FA) in plasma and for this reason, it has been postulated its involvement in the establishment of resistance. However, the mechanisms involved are not completely known. Recent studies are suggestive that the saturated fatty acids can impair mitochondrial function in skeletal muscle cells. Thus, this project will evaluate if insulin resistance induced by high-fat diet is related to mitochondrial dysfunction in skeletal muscle. For this, rats will be submitted to the administration of high-fat diet during three weeks. After this period, analysis of the mitochondrial function and expression of genes involved in mitochondrial biogenesis and metabolism will be carried out. (AU)