Synthesis, characterization and application of tripodal ligands with selectivity for Cu(II) in the cellular environment

• Grant number: 12/06754-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Effective date (Start): October 01, 2012
• Effective date (End): April 30, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Giselle Cerchiaro
• Grantee: Andreza Cândido Matias
• Supervisor: Pascale Delangle
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Research place: Commissariat à l'énergie atomique et aux énergies alternatives (CEA), France
• Associated to the scholarship: 11/22949-7 - Effects of copper and zinc unbalance on cell viability and ROS generation in neuroblastoma cell culture, BP.DD

Abstract

This project corresponds to a PhD stage for the student Andreza Candido Matias. In her PhD work, the student has used different commercial chelating agents for copper and zinc, which were membrane permeable. The objective was to study the effects of the imbalance of these metals on cell viability or death and generation of reactive oxygen species (ROS) in cells in culture.She obtained promising results during her work in Brazil and therefore we are planning a collaboration with the research group of Dr Pascale Delangle in Grenoble, France. Novel copper(II) tripodal ligands, will be designed and synthesized to get more efficient chelating agents than the commercially available ones, which would be able to cross the plasma membrane and chelate copper (II) intracellularly. The student will learn from the expertise of the French group to synthesize and purify these tripodal-type copper (II) ligands, and upon returning to Brazil after 7 months of internship, these ligands will be used in cell cultures to finalize this project. Also a profitable collaboration will be established, with also a French student interchange and a visit of Dr Pascale Delangle to Brazil. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONTE-DABAN, AMANDINE; BOFF, BASTIEN; MATIAS, ANDREZA CANDIDO; APARICIO, CLAUDIA N. MONTES; GATEAU, CHRISTELLE; LEBRUN, COLETTE; CERCHIARO, GISELLE; KIEFFER, ISABELLE; SAYEN, STEPHANIE; GUILLON, EMMANUEL; et al. A Trishistidine Pseudopeptide with Ability to Remove Both Cu-I and Cu-II from the Amyloid-beta Peptide and to Stop the Associated ROS Formation. CHEMISTRY-A EUROPEAN JOURNAL, v. 23, n. 67, p. 17078-17088, DEC 1 2017. (12/06754-4)