Genomic profile study of carcinoma ex-pleomorphic adenoma by array-comparative genomic hybridization

• Grant number: 11/23366-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2012
• End date: June 30, 2015
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Albina Messias de Almeida Milani Altemani
• Grantee: Fernanda Viviane Mariano Brum Corrêa
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Carcinoma ex pleomorphic adenoma (CXPA) is an aggressive neoplasm, and its pathogenesis is still unclear, although it is believed to result from the accumulation of genetic alterations in pleomorphic adenomas (PAs) with long duration. In this project we will study a group of CXPAs composed of tumors in different stages of carcinogenesis (intracapsular, minimally and frankly invasive), and classified according to histologic subtypes by array-comparative genomic hybridization (array-CGH). The literature review shows that this technique has been used to explore chromosomal changes only in a few cases of CXPA. Array-CGH will allow us a global analysis of changes in chromosome copy number in a representative sample, unlike the traditional cytogenetic techniques. Tumor cells have multiple gains and losses of whole chromosomes or arms, therefore, the array-CGH can potentially reveal copy number changes in chromosomal locus unexplored. The study of a group of CXPA in different stages of carcinogenesis with this technique will enable the identification of changes in copy number of chromosomal regions associated with tumor progression. The search for sites of genes involved in tumor progression may provide prognostic indicators of clinical disease. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARIANO, FERNANDA VIVIANE; FIDALGO, FELIPE; MAION CASARIM, ANDRE LUIS; MARTINS, ANTONIO SANTOS; SCARINI, JOAO FIGUEIRA; DE LIMA SOUZA, REYDSON ALCIDES; EGAL, ERIKA SAID; KOWALSKI, LUIZ PAULO; VICTORINO KREPISCHI, ANA CRISTINA; ALTEMANI, ALBINA. Somatic copy number alterations in pleomorphic adenoma and recurrent pleomorphic adenoma. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 129, n. 1, p. 59-64, JAN 2020. (15/07304-0, 17/00831-0, 11/23204-5, 11/23366-5)

DE BRITO, BEATRIZ SAMARA; GIOVANELLI, NATALIA; EGG, ERIKA SAID; SANCHEZ-ROMERO, CELESTE; DO NASCIMENTO, JULIANA DE SOUZA; MARTINS, ANTONIO SANTOS; TINCANI, ALFIO JOSE; DEL NEGRO, ANDRE; GONDAK, ROGERIO DE OLIVEIRA; DE ALMEIDA, OSLEI PAES; et al. Loss of expression of Plag1 in malignant transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma. HUMAN PATHOLOGY, v. 57, p. 152-159, NOV 2016. (14/18312-1, 11/23204-5, 11/23366-5)

FERNANDA VIVIANE MARIANO; ANA FLÁVIA COSTA; ROGÉRIO OLIVEIRA GONDAK; ANTONIO SANTOS MARTINS; ANDRÉ DEL NEGRO; ÁLFIO JOSÉ TINCANI; ALBINA ALTEMANI; OSLEI PAES DE ALMEIDA; LUIZ PAULO KOWALSKI. Cellular Proliferation Index between Carcinoma Ex-Pleomorphic Adenoma and Pleomorphic Adenoma. Brazilian Dental Journal, v. 26, n. 4, p. 416-421, 2015-08-00. (11/23366-5, 11/23204-5)

FERNANDA VIVIANE MARIANO; LUIS FERNANDO VIDAL SACCOMANI; KARINA GIOVANETTI; ANDRÉ DEL NEGRO; LUIZ PAULO KOWALSKI; ANA CRISTINA VICTORINO KREPISCHI; ALBINA ALTEMANI. Perfil genômico de um carcinoma espinocelular ex-adenoma pleomórfico comparado a um carcinoma espinocelular de cabeça e pescoço. Brazilian Journal of Otorhinolaryngology, v. 84, n. 3, p. 393-397, 2018-06-00. (11/23366-5, 11/23204-5)

FERNANDA VIVIANE MARIANO; KARINA GIOVANETTI; LUIS FERNANDO VIDAL SACCOMANI; ANDRÉ DEL NEGRO; LUIZ PAULO KOWALSKI; ANA CRISTINA VICTORINO KREPISCHI; ALBINA ALTEMANI. Carcinoma ex-adenoma pleomórfico derivado de adenoma pleomórfico recorrente mostra diferença importante por array CGH em comparação com adenoma pleomórfico recorrente sem transformação maligna. Brazilian Journal of Otorhinolaryngology, v. 82, n. 6, p. 687-694, 2016-12-00. (11/23366-5, 11/23204-5)

MARIANO, FERNANDA VIVIANE; EGAL, ERIKA SAID; PRAMIO, DIMITRIUS; FIDALGO, FELIPE; SARA, ERICA; COSTA, ANA FLAVIA; GONDAK, ROGERIO DE OLIVEIRA; DELLA COLETTA, RICARDO; DE ALMEIDA, OSLEI PAES; KOWALSKI, LUIZ PAULO; et al. Evaluation of a subset of tumor suppressor gene for copy number and epigenitic changes in pleomorphic adenoma and carcinoma ex-pleomorphic adenoma carcinogenesis. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 122, n. 3, p. 322-331, SEP 2016. (11/23204-5, 11/23366-5)

MARIANO, FERNANDA VIVIANE; RINCON, DANIEL; GONDAK, ROGERIO OLIVEIRA; JORGE, ROGERIO; LOPES, MARCIO AJUDARTE; ALTEMANI, ALBINA; DE ALMEIDA, OSLEI PAES; KOWALSKI, LUIZ PAULO. Carcinoma ex-pleomorphic adenoma of upper lip showing copy number loss of tumor suppressor genes. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 116, n. 1, p. 69-74, JUL 2013. (11/23204-5, 11/23366-5)

MARIANO, F. V.; NORONHA, A. L. F.; GONDAK, R. O.; ALTEMANI, A. M. DE A. M.; DE ALMEIDA, O. P.; KOWALSKI, L. P.. Carcinoma ex pleomorphic adenoma in a Brazilian population: clinico-pathological analysis of 38 cases. International Journal of Oral and Maxillofacial Surgery, v. 42, n. 6, p. 685-692, JUN 2013. (11/23204-5, 11/23366-5)

MARIANO, FERNANDA VIVIANE; DOS SANTOS, HARIM TAVARES; DELGADO AZANERO, WILSON; DA CUNHA, ISABELA WERNECK; COUTINHO-CAMILO, CLAUDIA MALHEIROS; DE ALMEIDA, OSLEI PAES; KOWALSKI, LUIZ PAULO; ALTEMANI, ALBINA. Mammary analogue secretory carcinoma of salivary glands is a lipid-rich tumour, and adipophilin can be valuable in its identification. Histopathology, v. 63, n. 4, p. 558-567, OCT 2013. (12/18086-6, 11/23204-5, 11/23366-5, 12/18104-4)