Protein and metabolic profiles of serum and plasma from sugar cane workers: biological and analytical variability

• Grant number: 12/05026-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2012
• Effective date (End): June 30, 2013
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Eloiza Helena Tajara da Silva
• Grantee: Adil Bachir Fares
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The National Alcohol Program (Proálcool) is a successful Brazilian renewable fuel initiative aiming to reduce the country's oil dependence. The program has shown positive results although accompanied by potential damage: the environmental impact mainly derives from the particulate matter emissions due to sugarcane burning, which is potentially harmful to human health, and the repetitive and exhaustive physical activity of sugarcane workers. Considering the influence of biological and analytical variability in the data obtained by metabolomic and proteomic approaches, this study aims to evaluate the reproducibility and variability of protein and metabolite profiles from serum and plasma obtained from sugar cane workers using specific protocols for blood collection and processing. (AU)