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Neural Mobilization: molecular and behavioral assessment in rats after induction of neuropathic pain

Grant number: 12/05840-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2012
Effective date (End): June 30, 2015
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal researcher:Marucia Chacur
Grantee:Fabio Martinez dos Santos
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Neural Mobilization (MOB) is a noninvasive method which has been clinically shown to be effective in reduction of pain sensitivity. This study aims to examine the effects of MOB on the pain sensitivity induced by chronic constriction injury (CCI) of sciatic nerve of rats and in animals with iontophoretic lesion of ventrolateral periaqueductal gray (vl PAG) by stereotactic technique. CCI was performed according to the technique described by Bennett and Xie (1988) and PAG lesion was performed as described in materials and methods. Both surgery were performed in adult male rats, subsequently the animals were submitted to ten MOB sessions initiated 14 days after CCI injury. In addition, we will also evaluate the locomotion behavioral through sciatic nerve functional index (SFI) and the maximal tetanic muscle force of tibialis anterior muscle using contractile function. After treatments with MOB and behavioral tests, the animals will be euthanized and tissues such as sciatic nerve, ganglia posterior roots (L4-L6) and PAG will be removed. The sciatic nerve will be used for analysis their ultrastructure through electron microscopy. Root ganglia (DRG's L4-L6) and PAG will be processed by Western blot test for the detection of opioid receptors, substance P and TRPV receptor. Our preliminary results concerning the test of the sciatic nerve functional index (SFI) showed an improvement in the treated group (CCI MOB) when compared to the untreated group (CCI). Similarly, our preliminary results on the maximal tetanic muscle showed a decrease of 52% in the untreated group (CCI) when compared with control animals (naïve) and an increase of 172% of the force in treated group (CCI MOB) when compared to the untreated group (CCI). However, animals with lesions in vl PAG and neuropathic pain when submitted to the treatment of Neural Mobilization showed a slight tendency of reversal in mechanical hyperalgesia. Thus, we suggest that Neural Mobilization reverses CCI-induced locomotor dysfunction, and increased 172% to maximum tetanic force of the tibialis anterior muscle compared to the untreated group. Moreover, there seems to be a strong influence of vl PAG in pain control, since the rats with lesions of the vl PAG not responded to treatments with Neural Mobilization.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, MARA EVANY; DA SILVA, JOYCE TEIXEIRA; BRIOSCHI, MARCOS LEAL; CHACUR, MARUCIA. Effects of photobiomodulation therapy on neuropathic pain in rats: evaluation of nociceptive mediators and infrared thermography. Lasers in Medical Science, v. 36, n. 7, p. 1461-1467, SEP 2021. Web of Science Citations: 0.
MARTINS, DANIEL OLIVEIRA; DOS SANTOS, FABIO MARTINEZ; CIENA, ADRIANO POLICAN; WATANABE, II-SEI; DE BRITTO, LUIZ ROBERTO G.; DIAS LEMOS, JOSE BENEDITO; CHACUR, MARUCIA. Neuropeptide expression and morphometric differences in crushed alveolar inferior nerve of rats: Effects of photobiomodulation. Lasers in Medical Science, v. 32, n. 4, p. 833-840, MAY 2017. Web of Science Citations: 4.
OLIVEIRA, M. E.; SANTOS, F. M.; BONIFACIO, R. P.; FREITAS, M. F.; MARTINS, D. O.; CHACUR, M. Low level laser therapy alters satellite glial cell expression and reverses nociceptive behavior in rats with neuropathic pain. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 16, n. 4, p. 547-554, APR 1 2017. Web of Science Citations: 6.
GIARDINI, ALINE CAROLINA; DOS SANTOS, FABIO MARTINEZ; DA SILVA, JOYCE TEIXEIRA; DE OLIVEIRA, MARA EVANY; MARTINS, DANIEL OLIVEIRA; CHACUR, MARUCIA. Neural Mobilization Treatment Decreases Glial Cells and Brain-Derived Neurotrophic Factor Expression in the Central Nervous System in Rats with Neuropathic Pain Induced by CCI in Rats. PAIN RESEARCH & MANAGEMENT, 2017. Web of Science Citations: 3.
SANTOS, FABIO MARTINEZ; GRECCO, LEANDRO HENRIQUE; PEREIRA, MARCELO GOMES; OLIVEIRA, MARA EVANY; ROCHA, PRISCILA ABREU; SILVA, JOYCE TEIXEIRA; MARTINS, DANIEL OLIVEIRA; MIYABARA, ELEN HARUKA; CHACUR, MARUCIA. The neural mobilization technique modulates the expression of endogenous opioids in the periaqueductal gray and improves muscle strength and mobility in rats with neuropathic pain. Behavioral and Brain Functions, v. 10, MAY 13 2014. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTOS, Fabio Martinez dos. Neural mobilization: molecular and behavioral assessment in rats after induction of neuropathic pain.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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