Protein stability evaluation of transcription factor AtbZIP63 in biotic/abiotic stress responses and the role of this gene in resistance of Arabidopsis thaliana to Pseudomonas syringae pv tomato strain DC3000

• Grant number: 12/09351-8
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2012
• Effective date (End): September 30, 2016
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal researcher: Michel Georges Albert Vincentz
• Grantee: Cleverson Carlos Matiolli
• Home Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 08/52071-0 - Energetic homeostasis and sugar signaling: diversification of the molecular mechanisms involved in the control of the energetic balance in angiosperms, AP.BIOEN.TEM
• Associated scholarship(s): 13/07295-6 - Assessing the role of the transcription factor AtbZIP63 in Arabidopsis thaliana resistance to Pseudomonas syringae pv. tomato strain DC3000, BE.EP.PD

Abstract

In the model plant Arabidopsis thaliana, the mRNA accumulation of AtbZIP63 transcription factor is regulated by daily fluctuations of sugars, absicisic acid (ABA), circadian clock and pathogen infection. In agreement with these data, the functional classification of 280 misregulated genes in atbzip63-1 mutant suggest that AtbZIP63 is directly or indirectly involved in regulation of genes related to energy starvation, abiotic and biotic stresses, probably modulating the energy use in response to environmental challenges. To extend the knowledge about AtbZIP63 role, we perform the search for AtbZIP63 interacting proteins using the Yeast-two hybrid (Y2H) approach, and the results had shown an enrichment of protein degradation related genes. Moreover, preliminary analysis showed that atbzip63-1 mutant is more resistant to Pseudomonas syringae pv. tomato (Pst) strain DC3000. This research project aims to establish the mechanisms of AtbZIP63 protein stability in response to energy deprivation and biotic and abiotic stresses. In a second step, elucidate the molecular mechanisms by which AtbZIP63 operates in plant defense against Pst DC3000 in collaboration with Dr. Maeli Melotto of University of Texas at Arlington (UTA). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA, AMERICO J. C.; MATIOLLI, CLEVERSON C.; NEWMAN, DAVID W.; VIEIRA, JOAO G. P.; DUARTE, GUSTAVO T.; MARTINS, MARINA C. M.; GILBAULT, ELODIE; HOTTA, CARLOS T.; CALDANA, CAMILA; VINCENTZ, MICHEL. The sugar-responsive circadian clock regulator bZIP63 modulates plant growth. NEW PHYTOLOGIST, v. 231, n. 5, p. 1875-1889, SEP 2021. Web of Science Citations: 1.

VIANA, AMERICO J. C.; MATIOLLI, CLEVERSON C.; NEWMAN, DAVID W.; VIEIRA, JOAO G. P.; DUARTE, GUSTAVO T.; MARTINS, MARINA C. M.; GILBAULT, ELODIE; HOTTA, CARLOS T.; CALDANA, CAMILA; VINCENTZ, MICHEL. The sugar-responsive circadian clock regulator bZIP63 modulates plant growth. NEW PHYTOLOGIST, JUN 2021. Web of Science Citations: 0.