Reproductive indicators of Nile Tilapia, Oreochromis niloticus fed diets supplemented with probiotic composed of Bacillus subtilis

• Grant number: 12/13741-6
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: September 13, 2012
• End date: December 12, 2012
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maria José Tavares Ranzani de Paiva
• Grantee: Danielle de Carla Dias
• Supervisor: Miguel Angel Moriñigo Gutierrez
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). São Paulo , SP, Brazil
• Institution abroad: Universidad de Málaga (UMA), Spain
• Associated to the scholarship: 10/11694-5 - Reproductive indicators of tilapia, Oreochromis niloticus, feed with probiotic composed by Bacillus subtilis, BP.PD

Abstract

The proteomics is the study of the expression of the proteome of an organism, or the outcome of the expression of their genes, which allows us to understand the structural and functional effects caused by factors environment, including nutrition and in this case, probiotics. The two-dimensional electrophoresis, which combines separation of proteins according to their isoelectric point and, by its molecular weight, followed the identification of proteins by mass spectrometry is used for the method further studies proteomics. The electrophoresis in denaturing gradient gel (DGGE) is a separation method based on differences in electrophoretic behavior of denaturation of DNA fragments chain twofold. The DGGE is a powerful technique of genetic analysis that can be used to directly detect modification of a single base and polymorphisms in genomic DNA, DNA amplified DNA and clone PCR. This technique will allow tracking of bacteria in the intestines of fish and possible changes in intestinal flora caused by probiotic supplementation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANIELLE DE CARLA DIAS; FERNANDA DE PAIVA BADIZ FURLANETO; FÁBIO ROSA SUSSEL; LEONARDO TACHIBANA; GIOVANI SAMPAIO GONÇALVES; CARLOS MASSATOSHI ISHIKAWA; MARIENE MIYOKO NATORI; MARIA JOSÉ T. RANZANI-PAIVA. Economic feasibility of probiotic use in the diet of Nile tilapia, Oreochromis niloticus, during the reproductive period. Acta Sci., Anim. Sci., v. 42, 2020-02-03. (12/13741-6, 10/11694-5, 10/11141-6, 13/19502-6)