Production of L (+)-lactic acid by Lactobacillus casei Ke11 using industrial waste.

• Grant number: 11/16575-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2012
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Jonas Contiero
• Grantee: Paola Monteiro de Oliveira
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Production of lactic acid by fermentation need for pH control the calcium carbonate and produce calcium sulfate as a waste. In this project is proposed a simple alimentation method, controlled by the pH decrease (pH stat) during the lactic acid production, in order to increase the fermentation's efficiency and prevent the formation of gypsum. The microorganisms L(+) latic acid producer, Lactobacillus casei Ke11 potentials producers will be used. First, experiments will be done for the better culture medium of production determination, different waste will be available, like cheese whey, raw cane sugar, corn steep liquor. After the determination of the better culture medium, will be realized the factorial central composite design with four replicas at the midpoint. With the optimization product, will be available two types of fed batch, pH-stat and exponential. In all this experiments will be withdraw samples, after 48 hours of fermentation, for lactic acid production, biomass and substrate consumption available. Moreover, in order to compare the different types of process (fed and simple batch) will be held on the fermentation kinetics in bioreactors.Keywords: fed batch, Lactobacillus sp., optimization, purification, agro-industrial residues.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Paola Monteiro de. Produção de L (+)-ácido lático por Lactobacillus casei Ke11 utilizando insumos agroindustriais. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.