Biodiversity of marine and terrestrial yeasts from Antarctic ecossystems and prospection of lipases

• Grant number: 10/08352-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2012
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lara Durães Sette
• Grantee: Alysson Wagner Fernandes Duarte
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil

Abstract

The Antarctic continent is characterized by restrictive environmental conditions of climate, habitats and biogeography. The main organisms adapted to the conditions of this environment are the micro-organisms, in particular highlighted the yeasts, with potential application in several biotechnological processes. Among the enzymatic arsenal of fungi stand out lipases (EC. 3.1.1.3), hydrolases that have wide industrial applicability because of the versatility in its molecular structure and their catalytic properties. Moreover, the microbial biodiversity in this environment is only in early stages of knowledge. Thus, in order to expand knowledge of the role of these micro-organisms in different Antarctic environments and the production and application of lipases in processes that require low and moderate temperatures and / or high salt concentrations, this project has as main objectives: 1) Evaluate the diversity yeast from different samples (marine and terrestrial) from the Antarctica by isolation and culture, 2) Prospection of lipase produced at low and moderate temperatures and / or saline conditions by the yeast-derived Antarctica 3) Optimize lipase production (by means of statistical, software STATISTICA 7.0), 4) Purify and characterize biochemically active lipases in low and moderate temperatures, 5) Identify the functional genes with lipolytic activity by classical PCR, 6) Evaluate the activity of lipolytic genes by real time PCR; in addition, the yeasts that are experiencing biotechnological potential or that are representatives of biodiversity Antarctica, after the taxonomic characterization will be deposited at the collection thematic Micro-organisms of Antarctica structured within the Collection Brasileira of Micro-organisms Environment and Industry (CBMAI).

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, SI, p. 874-885, OCT 2016. (10/17033-0, 13/19486-0, 10/08352-5)

DUARTE, A. W. F.; DAYO-OWOYEMI, I.; NOBRE, F. S.; PAGNOCCA, F. C.; CHAUD, L. C. S.; PESSOA, A.; FELIPE, M. G. A.; SETTE, L. D.. Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples. EXTREMOPHILES, v. 17, n. 6, p. 1023-1035, NOV 2013. (10/17033-0, 10/08352-5)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, p. 12-pg., 2016-10-01. (13/19486-0, 10/08352-5, 10/17033-0)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, p. 11-pg., 2015-12-17. (13/19486-0, 10/08352-5, 10/17033-0)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, n. 2, p. 215-225, DEC 17 2015. (10/17033-0, 13/19486-0, 10/08352-5)

FERNANDES DUARTE, ALYSSON WAGNER; BONUGLI-SANTOS, RAFAELLA COSTA; FERRAREZI DUARTE, ANA LUCIA; GOMES, ELENI; SETTE, LARA DURAES. Statistical experimental design applied to extracellular lipase production by the marine Antarctic yeast Leucosporidium scottii CRM 728. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 32, MAR 2021. (13/19486-0, 10/08352-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DUARTE, Alysson Wagner Fernandes. Biodiversity of yeasts from marine and terrestrial antarctic ecosystems and prospection of lipases.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.