MORPHOLOGICAL AND MOLECULAR RESPONSES IN SKELETAL MUSCLE OF AGED RATS SUBMITTED TO PHYSICAL TRAINING AFTER ATROPHYC STIMULUS

• Grant number: 11/14484-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2012
• End date: November 30, 2014
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Maeli Dal Pai
• Grantee: Ivan José Vechetti Júnior
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 12/20183-0 - Role of miR-1 in the mTORC1 signaling pathway in skeletal muscle, BE.EP.DR

Abstract

Recent research advances in understanding the mechanisms and signaling pathways involved in the processes of recovery of muscle mass in old age, using different stimuli. These studies can contribute to the development of strategies to attenuate or block the loss of muscle mass associated with aging, such as physical training. In this context, several myogenic factors and signaling pathways that control the activity of satellite cells have been studied to further elucidate the events involved in the recovery process of muscle mass during aging. Among them stand out from the myogenic regulatory factors (MRFs), the hypertrophic pathway components (factor insulin-like growth (IGF-I), and myostatin (MSTN), which have been described as potential mediators of muscle growth. Thus, the purpose of this study is to assess the morphological and molecular responses of skeletal muscle of senile rats subjected to physical training after stimulation of atrophy. Therefore, will be used 64 male Wistar rats (18 months), divided into 8 groups (n = 8): C: control animals a week, I: Animals immobilized a week, C3: control animals 3 days; R3: Animals immobilized and recovered for 3 days, T3: Animals immobilized and submitted to exercise for 3 days; C7: Animals controls 7 days; R7: Animals immobilized and subsequently recovered by 7 days, T7: Animals immobilized and subsequently subjected to exercise for seven days. Initially, the animals in groups I, R3, R7, T3 and T7, will undergo a period of 7 days of immobilization of the hind limb. Muscle atrophy will be confirmed after a direct statistical comparison of the values of cross-sectional area (CSA) of muscle fibers studied in animals in groups I and C, sacrificed immediately after the immobilization period. Then the T3 and T7 groups, will undergo a rehabilitation program with muscle aerobic exercise (swimming) within 3 and 7 days respectively. The groups C, C3 and C7 are kept without any stimulus atrophic and not be subjected to exercise. At the end of the experiment the animals are sacrificed and the plantaris muscle removed and then subjected to morphological analysis, immunohistochemistry, and molecular biochemistry. The data will be submitted to appropriate statistical analysis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VECHETTI-JUNIOR, IVAN J.; BERTAGLIA, RAQUEL S.; FERNANDEZ, GEYSSON J.; DE PAULA, TASSIANA G.; DE SOUZA, RODRIGO W. A.; MORAES, LEONARDO N.; MARECO, EDSON A.; DE FREITAS, CARLOS E. A.; AGUIAR, ANDREO F.; CARVALHO, ROBSON F.; et al. Aerobic Exercise Recovers Disuse-induced Atrophy Through the Stimulus of the LRP130/PGC-1 alpha Complex in Aged Rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 71, n. 5, p. 601-609, MAY 2016. (11/14484-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VECHETTI JÚNIOR, Ivan José. Resposta morfológicas e moleculares do músculo esquelético de ratos idosos submetidos ao treinamento físico após estímulo atrófico. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.