Inhibitors Recognition and Flexibility of Cdc25B Phosphatase

• Grant number: 12/00543-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2012
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Guilherme Menegon Arantes
• Grantee: Raphael Santa Rosa Sayegh
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Cdc25 phosphatases act on cell cycle checkpoints. Several kinds of tumors overexpress this enzyme.Therefore, the study of Cdc25s inhibitors can aid the development of new anticancer therapies. Several low specificity inhibitors have been already described for Cdc25B, isoform which crystal structure of the catalytic domain has been determined by X-ray diffraction. Computational data indicate high flexibilityin the C-terminus that can influence competitive inhibitor binding. In this project, we aim to evaluate and extend these simulations using structural and dynamical measures of the protein in solution obtained by nuclear magnetic resonance. This data will be used to construct new conformational ensembles. The ensembles will be used in docking experiments with inhibitors and the results compared with experimental datafrom the same Cdc25B+inhibitor complexes. Thus, the influence of the protein flexibility in the molecular recognition will be evaluated. This project can lead to important advances in methods used for development of new ligands to flexible or partially unfolded protein.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REIS, ANDRE A. O.; SAYEGH, RAPHAEL S. R.; MARANA, SANDRO R.; ARANTES, GUILHERME M.. Combining Free Energy Simulations and NMR Chemical-Shift Perturbation To Identify Transient Cation-pi Contacts in Proteins. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 60, n. 2, p. 890-897, FEB 2020. (18/25952-8, 16/22365-9, 16/24096-5, 12/00543-1, 18/08311-9)

SAYEGH, RAPHAEL S. R.; TAMAKI, FABIO K.; MARANA, SANDRO R.; SALINAS, ROBERTO K.; ARANTES, GUILHERME M.. Conformational flexibility of the complete catalytic domain of Cdc25B phosphatases. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, v. 84, n. 11, p. 1567-1575, NOV 2016. (14/19439-5, 08/55914-9, 13/17883-2, 14/21900-2, 12/00543-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAYEGH, Raphael Santa Rosa. Conformational flexibility of the catalytic domain of Cdc25B phosphatase. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.