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Determination of the composition of the acquired pellicle formed on enamel and dentin using proteomic techniques

Grant number: 12/10452-3
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): September 27, 2012
Effective date (End): January 26, 2013
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Marília Afonso Rabelo Buzalaf
Grantee:Melina Rodrigues Bellini
Supervisor abroad: Walter Luiz Siqueira
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: Western University , Canada  
Associated to the scholarship:10/13865-1 - Determination of the composition of the acquired pellicle formed on enamel and dentin using proteomic techniques, BP.MS

Abstract

This study will be comparing the differential protein expression in acquired pellicule formed in situ, at different times (10 minutes and 2 hours) on the enamel and the dentin. The samples were divided into two groups: "pool" (9 samples of subjects) and group individuals (samples from each of the 9 volunteers). The subjects were device mandibular in the oral cavity for film formation obtained, the blocks were removed and the film was removed with a filter paper soaked in 3% citric acid. After protein extraction, the same will be submitted to fractionation by ion exchange chromatography. The collected fractions are concentrated and subjected to reverse phase liquid chromatography interfaced with mass spectrometry (LC-ESI-MS/MS). The data obtained from MS / MS will be processed and submitted jointly to the program SQUEST (Bioworks Browser 3.2, Thermo-Finnigan, San Jose, CA, USA). The searches will be conducted using the databases SWISS-PROT and TrEMBL. (AU)

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